Transcriptome profiling revealed novel transcriptional regulators in maize responses to Ostrinia furnacalis and jasmonic acid.
Transcriptome profiling revealed novel transcriptional regulators in maize responses to Ostrinia furnacalis and jasmonic acid.
复制标题
转录组分析揭示了玉米对亚洲玉米螟和茉莉酸反应中的新型转录调节因子
DOI：
10.1371/journal.pone.0177739
复制
发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Lang Z
中科院分区：
文献类型：
--
作者：
Wang H;Li S;Teng S;Liang H;Xin H;Gao H;Huang D;Lang Z
咀嚼昆虫在世界范围内造成农作物生产的严重产量损失。农作物通过转录促进一系列对害虫的天敌有毒或有吸引力的防御基因产物来抵抗咀嚼昆虫。然而，转录重编程在植物对咀嚼昆虫防御反应中的复杂性仍然没有得到很好的理解。本研究利用RNA-Seq技术分析了玉米幼苗对亚洲玉米螟（Asian corn borer，Ostrinia acalis）和茉莉酸（jasmonic acid，JA）的全基因组早期反应。聚类的差异表达的基因（DEG）沿着与功能富集分析显示重要的生物过程中调节响应ACB侵染和/或茉莉酸。此外，具有不同表达模式的DEG在其启动子上具有不同的顺式元件家族。还分析了每个DEG组中差异表达转录因子（DETF）的多个库存。利用转基因玉米原生质体瞬时表达技术研究了DETF在玉米防御反应和JA信号转导中的潜在作用，结果表明ZmNAC 60是一个ACB和JA诱导的DETF，是一个新的JA和防御途径基因的正调控因子。本研究为玉米防御反应和JA信号传导的早期动态提供了全面的转录图像，DETF的鉴定为进一步研究玉米防御反应中主调节因子的功能基因组学提供了潜在的靶点。
Chewing insects cause severe yield losses in crop production worldwide. Crop plants counteract chewing insects by transcriptionally promoting a repertoire of defense gene products that are either toxic to, or attractive to the natural enemies of, pest insects. However, the complexity of the transcriptional reprogramming in plant defense response against chewing insects is still not well understood. In this study, the genome-wide early responses in maize seedlings to Asian corn borer (ACB, Ostrinia furnacalis) and also to jasmonic acid(JA), the pivotal phytohormone controlling plant defense response against herbivory, were transcriptionally profiled by RNA-Seq. Clustering of differentially expressed genes (DEGs) along with functional enrichment analysis revealed important biological processes regulated in response to ACB infestation and/or jasmonic acid. Moreover, DEGs with distinct expression patterns were differentially enriched with diverse families of cis-elements on their promoters. Multiple inventories of differentially expressed transcription factors (DETFs) in each DEG group were also analyzed. A transient expression assay using transfected maize protoplastswas established to examine the potential roles of DETFs in maize defense response and JA signaling, and this was used to show that ZmNAC60, an ACB- and JA-inducible DETF, represented a novel positive regulator of JA and defense pathway genes. This study provided a comprehensive transcriptional picture for the early dynamics of maize defense responses and JA signaling, and the identification of DETFs offered potential targets for further functional genomics investigation of master regulators in maize defense responses against herbivory.