Discontinuous epitope prediction based on mimotope analysis
Discontinuous epitope prediction based on mimotope analysis
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DOI：
10.1093/bioinformatics/btl012
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发表时间：
2006-05-01
期刊：
影响因子：
5.8
通讯作者：
Molina, F
中科院分区：
文献类型：
--
作者：
Moreau, V;Granier, C;Molina, F
动机：噬菌体展示是用于获得肽模拟表位的广泛技术，所述肽模拟表位通过以与天然表位类似的方式结合到给定的单克隆抗体来选择。然而，由抗原表面上的模拟表位模拟的相互作用位点的定位并不总是一个简单的任务。MIMOP是一个计算工具，其目的是帮助实验者分析一组模拟表位序列，并指导他们在识别的mimickedregion.Results：为了预测潜在的表位区域，MIMOP集成了两种不同的方法相结合的二维和三维分析：MimAlign开始从退化的比对分析，和MimCons是基于共识identification。该工具的相关性和实用性说明了四个用例对应于现实生活中的情况。
Motivation: Phage display is a widespread technique used to obtain peptide mimotopes selected by binding to a given monoclonal antibody in a similar way as the native epitope. However, the localization of the interaction site mimicked by the mimotopes on the surface of the antigen is not always a straightforward task. MIMOP is a computational tool developed with the aim of helping experimentalists to analyze a set of mimotope sequences and guide them in the identification of the mimicked region.Results: To predict potential epitopic regions, MIMOP integrates two different approaches combining two- and three-dimensional analyses: MimAlign starts from degenerated alignment analyses, and MimCons is based on consensus identification. The relevance and usefulness of the tool are illustrated by four use cases corresponding to real-life situations.