Monoclonal antibodies to the extracellular glucosyltransferases from Streptococcus sobrinus 6715.
Monoclonal antibodies to the extracellular glucosyltransferases from Streptococcus sobrinus 6715.
复制标题
来自 Sobrinus 6715 的胞外葡萄糖基转移酶的单克隆抗体。
DOI：
10.1128/iai.55.8.1900-1905.1987
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发表时间：
1987
影响因子：
3.1
通讯作者：
Stein,J
中科院分区：
文献类型：
--
作者：
McCabe,MM;Alberts,M;Stein,J
Murine monoclonal antibodies (MAbs) were raised against the glucosyltransferases (GTFs) of Streptococcus sobrinus 6715. The antibody panels included MAbs raised against the primer-independent, soluble product enzyme (GTF-Si) which did not cross-react with other GTFs, as well as MAbs raised against the primer-dependent, soluble product enzyme (GTF-Sd) which recognized both GTF-Si and GTF-Sd, thus indicating that these catalytically distinct enzymes share epitopes. MAbs raised against GTF-I recognized several forms of GTF-I and did not cross-react with the GTF-S enzymes. None of the MAbs recognized the major glucan-binding protein of S. sobrinus. Two MAbs inhibited glucan synthesis, one blocking primer synthesis by GTF-Si by 89% and the second inhibiting that by GTF-I by 92%.
