Different efficiencies of the tag and AlkA DNA glycosylases from Escherichia coli in the removal of 3-methyladenine from single-stranded DNA
Different efficiencies of the tag and AlkA DNA glycosylases from Escherichia coli in the removal of 3-methyladenine from single-stranded DNA
复制标题
DOI：
10.1016/s0014-5793(96)01166-0
复制
发表时间：
1996-11-11
期刊：
影响因子：
3.5
通讯作者：
Seeberg, E
中科院分区：
文献类型：
--
作者：
Bjelland, S;Seeberg, E
大肠杆菌具有两种不同的DNA修复糖基化酶Tag和AlkA，它们具有相似的从双链DNA去除烷基化产物3-甲基腺嘌呤的能力。在这项研究中，我们表明，这些酶有相当不同的活动，从单链DNA的3-甲基腺嘌呤切除，AlkA是10-20倍，比标签更有效。我们建议，AlkA和其他糖基化酶，以及可能有一个重要的作用，在切除的碱基损伤从单链区域瞬时形成的DNA在转录和复制。
Escherichia coli possesses two different DNA repair glycosylases, Tag and AlkA, which have similar ability to remove the alkylation product 3-methyladenine from double-stranded DNA. In this study we show that these enzymes have quite different activities for the excision of 3-methyladenine from single-stranded DNA, AlkA being 10-20 times more efficient than Tag. We propose that AlkA and perhaps other glycosylases as well may have an important role in the excision of base damage from single-stranded regions transiently formed in DNA during transcription and replication.