Gas chromatographic-mass spectrometric determination of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol for study on human urinary excretion of ginsenosides after ingestion of ginseng preparations
Gas chromatographic-mass spectrometric determination of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol for study on human urinary excretion of ginsenosides after ingestion of ginseng preparations
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DOI：
10.1016/s0378-4347(96)00304-0
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发表时间：
1997-02-21
影响因子：
3
通讯作者：
Eneroth, P
中科院分区：
文献类型：
--
作者：
Cui, JF;Bjorkhem, I;Eneroth, P
采用改进的气相色谱-质谱联用快速固相萃取法（GC-MS）研究了口服人参制剂后20(S)-原人参醇和20(S)-原人参醇苷在人体尿液中的排泄情况。以人参皂苷醇为内标，20(S)-原人参皂苷醇和20(S)-原人参皂苷醇在尿中释放后，采用选择离子监测的气相色谱-质谱联用技术，检测水平为几ng / mi尿。从尿液中提取人参皂苷的回收率大于80%，测定内变异系数小于5.0%。服用单剂量人参制剂后的结果表明，人参皂苷的摄入量与尿中排泄的20(S)-原嘌呤苷之间存在线性关系。约1.2%的剂量在5天内恢复。
An improved gas chromatographic-mass spectrometric method (GC-MS) with a fast solid-phase extraction on a newly introduced C-18 microcolumn, was applied to study the urinary excretion of 20(S)-protopanaxatriol and 20(S)-protopanaxatriol glycosides in man after oral administration of ginseng preparations. Using panaxatriol as internal standard, 20(S)-protopanaxadiol and 20(S)-protopanaxatriol (the aglycones of ginsenosides) could be determined at a detection level of a few ng per mi urine by GC-MS with selected-ion monitoring after their release from glycosides which occur in urine. The extraction recovery of ginsenosides from urine was more than 80% and the intra-assay coefficient of variation was less than 5.0%. The results after intake of single doses of ginseng preparations demonstrated a linear relation between the amounts of ginsenosides consumed and the 20(S)-protopanaxatriol glycosides excreted in urine. About 1.2% of the dose was recovered in five days.