Visualization of nucleic acids with synthetic exciton-controlled fluorescent oligonucleotide probes.
Visualization of nucleic acids with synthetic exciton-controlled fluorescent oligonucleotide probes.
复制标题
使用合成激子控制的荧光寡核苷酸探针对核酸进行可视化。
DOI：
10.1007/978-1-4939-2253-6_5
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Okamoto A
中科院分区：
文献类型：
--
作者：
Wang DO;Okamoto A
在识别靶核酸时适应新的光化学性质的工程化探针为DNA和RNA可视化技术提供了强大的工具。在这里，我们描述了一种快速有效的可视化方法，在固定和活细胞的核酸与杂交敏感的荧光寡核苷酸探针。这些探针在水性环境中由于荧光团之间的同二聚体、激子相互作用而被有效地猝灭，但在与具有互补序列的DNA或RNA杂交后变得高度荧光。新探针的快速杂交动力学和快速荧光激活允许应用简化常规荧光原位杂交方案并减少处理样品的时间量。此外，探针的杂交敏感荧光发射允许监测活细胞中RNA的动态行为。
Engineered probes to adapt new photochemical properties upon recognition of target nucleic acids offer powerful tools to DNA and RNA visualization technologies. Herein, we describe a rapid and effective visualization method of nucleic acids in both fixed and living cells with hybridization-sensitive fluorescent oligonucleotide probes. These probes are efficiently quenched in an aqueous environment due to the homodimeric, excitonic interactions between fluorophores but become highly fluorescent upon hybridization to DNA or RNA with complementary sequences. The fast hybridization kinetics and quick fluorescence activation of the new probes allow applications to simplify the conventional fluorescent in situ hybridization protocols and reduce the amount of time to process the samples. Furthermore, hybridization-sensitive fluorescence emission of the probes allows monitoring dynamic behaviors of RNA in living cells.