Identification of a male sterile geneMsinBrassica rapaL.
Identification of a male sterile geneMsinBrassica rapaL.
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芸苔属雄性不育基因Ms的鉴定。
DOI：
10.1007/s11032-020-01141-9
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发表时间：
2020
影响因子：
3.1
通讯作者：
Feng Hui
中科院分区：
文献类型：
--
作者：
Shi Fengyan;Liu Zhiyong;Wang Nan;Zhao Ying;Dong Shiyao;Zou Jiaqi;Feng Hui
甘蓝型油菜雄性不育系是制种的重要材料。在前期的研究中，我们获得了一个多等位基因遗传的大白菜雄性不育系。鉴定雄性不育基因MsinB。拉帕，我们用了B。拉帕尔。中国变种(‘AB03’；MSMS)和B.以紫花菜(‘AB04’；MsfMsf)为亲本，构建了一个F2分离群体，并将该基因定位于A07染色体上的单序列重复标记SSRY17和SSRY22之间，SSRY17和SSRY22分别位于基因两侧0.11和0.31 cM的遗传距离上。两个标记之间的间隔为231.3 kb，由21个基因组成。全基因组重测序和序列比较表明，只有一个基因Bra015018在所映射的区间内的一个外显子上含有插入-缺失(Indel)。该Indel由于终止密码子TGA的产生而导致翻译提前终止。Bra015018与AT1G34355同源，AT1G34355编码与植物生长和花发育相关的含有叉头相关(FHA)结构域的蛋白。基因分型表明，雄性不育与Indel完全共分离。QRT-PCR分析表明，Bra015018基因在雄蕊中特异表达。因此，我们预测Bra015018是MS的候选基因。
Male sterile lines are important for hybrid seed production inBrassica rapa. In our previous study, we obtained a multiple-allele inherited male sterile line of Chinese cabbage. To identify the male sterile geneMsinB. rapa, we usedB. rapaL. var.chinensis(‘AB03’;MsMs) andB. rapavar.purpuraria(‘AB04’;MsfMsf) as parents to construct an F2segregating population, and mapped theMsgene on chromosome A07 between single sequence repeat (SSR) markers SSRY17 and SSRY22, which flank the gene at genetic distances of 0.11 cM and 0.31 cM, respectively. The interval between the two markers was 231.3 kb and comprised 21 genes. Whole-genome resequencing and sequence comparison revealed that only one gene,Bra015018, harbored an insertion–deletion (InDel) in an exon in the mapped interval. This InDel led to premature termination of translation due to generation of the stop codon TGA.Bra015018was homologous toAT1G34355, which encoded a forkhead-associated (FHA) domain-containing protein that was related to plant growth and floral development. Genotyping revealed that male sterility was fully co-segregated with the InDel. qRT-PCR analysis showed thatBra015018was specifically expressed in the stamen. Consequently, we predicted thatBra015018was a candidate gene ofMs.