Serum concentrations and tissue expression of components of insulin-like growth factor-axis in females with type 2 diabetes mellitus and obesity: The influence of very-low-calorie diet
Serum concentrations and tissue expression of components of insulin-like growth factor-axis in females with type 2 diabetes mellitus and obesity: The influence of very-low-calorie diet
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DOI：
10.1016/j.mce.2012.04.005
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发表时间：
2012-09
影响因子：
4.1
通讯作者：
V. Toušková;P. Trachta;P. Kavalkova;J. Drápalová;D. Haluzíková;M. Mráz;Z. Lacinová;J. Marek;M. Haluzík
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文献类型：
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作者：
V. Toušková;P. Trachta;P. Kavalkova;J. Drápalová;D. Haluzíková;M. Mráz;Z. Lacinová;J. Marek;M. Haluzík
我们研究了 18 名健康女性、11 名肥胖非糖尿病女性 (OB) 和 13 名患有 2 型糖尿病 (T2DM) 的肥胖女性在基线和极低热量饮食 (VLCD) 后的皮下脂肪组织 (SCAT) 和外周单核细胞 (PM) 中胰岛素样生长因子 1 (IGF-1) 轴成分的血清浓度和 mRNA 表达。与对照组相比，T2DM 女性 SCAT 中 IGF-1、IGF-1 受体 (IGF-1R)、IGFBP-2（IGF 结合蛋白-2）和 IGFBP-3 表达降低，PM 中 IGF-1R 表达增加。 OB SCAT 中 IGF-1R 和 IGFBP-3 mRNA 表达与对照组相当。在 T2DM 女性中，VLCD 增加了 IGFBP-2 的血清水平和 SCAT 表达以及 IGFBP-3 的 PM 表达。我们得出结论，T2DM 受试者 SCAT 中 IGF-1、IGF-1R 和 IGFBP-3 表达减少以及 PM 中 IGF-1R 表达增加可能分别导致脂肪分化能力的变化和 PM 对亚临床炎症的调节。 PM 中 SCAT 和循环 IGFBP-2 和 IGFBP-3 的增加可能参与 VLCD 后代谢的改善。
We explored serum concentrations and mRNA expression of insulin-like-growth factor-1 (IGF-1) axis components in subcutaneous adipose tissue (SCAT) and peripheral monocytes (PM) of 18 healthy females, 11 obese non-diabetic females (OB) and 13 obese women with type 2 diabetes (T2DM) examined at baseline and after very-low-calorie diet (VLCD). T2DM women had decreased expression of IGF-1, IGF-1 receptor (IGF-1R), IGFBP-2 (IGF binding protein-2) and IGFBP-3 in SCAT and increased expression of IGF-1R in PM compared to control group. IGF-1R and IGFBP-3 mRNA expression in SCAT of OB was comparable to control group. In T2DM women VLCD increased serum levels and SCAT expression of IGFBP-2 and PM expression of IGFBP-3. We conclude that decreased IGF-1, IGF-1R and IGFBP-3 expression in SCAT and increased IGF-1R expression in PM of T2DM subjects might contribute to changes of fat differentiation capacity and to regulation of subclinical inflammation by PM, respectively. Increased SCAT and circulating IGFBP-2 and IGFBP-3 in PM might participate in metabolic improvements after VLCD.