The Effects of Template Rigidity and Amino Acid Type on Heterogeneous Calcium-Phosphate Mineralization by Langmuir Films of Amphiphilic and Acidic β-Sheet Peptides
The Effects of Template Rigidity and Amino Acid Type on Heterogeneous Calcium-Phosphate Mineralization by Langmuir Films of Amphiphilic and Acidic β-Sheet Peptides
复制标题
DOI：
10.1021/jp305386e
复制
发表时间：
2012-09-13
影响因子：
3.3
通讯作者：
Rapaport, Hanna
中科院分区：
文献类型：
--
作者：
Segman-Magidovich, Shlomit;Rapaport, Hanna
在由设计的两亲性和酸性β-折叠形成肽组成的系统中监测磷酸钙矿化，即Pro-Phe-(Asp-Phe)(5)-Pro (PFD-5)、Pro-Phe-(Glu-Phe)(5)-Pro (PFE-5)和Pro-Glu-(Phe-PSer)(4)-Phe-Glu-Pro （PPS）。这三种肽仅在亲水性氨基酸方面有所不同，因此，可以作为评估阴离子氨基酸类型对 β-折叠结构背景下矿化作用的良好模型。将单层肽沉积在模拟身体溶液 (SBF1.5) 上，并通过朗缪尔等温线测量、掠入射 X 射线衍射 (GIXD) 和布鲁斯特角显微镜 (BAM) 检测吸附矿物质随时间的影响。结果提供了对矿化过程中矿化膜形态和肽晶格行为的深入了解。研究发现肽模板的刚性以及氨基酸侧链的类型显着影响矿化形态和肽结构。这些结果将有助于更好地了解自然界中的磷酸钙矿化以及在骨组织再生应用的生物材料中的磷酸钙矿化。
Calcium-phosphate mineralization was monitored in systems composed of designed amphiphilic and acidic beta-sheet-forming peptides, namely Pro-Phe-(Asp-Phe)(5)-Pro (PFD-5), Pro-Phe-(Glu-Phe)(5)-Pro (PFE-5) and Pro-Glu-(Phe-PSer)(4)-Phe-Glu-Pro (PPS). The three peptides differ solely in terms of their hydrophilic amino acids and therefore, serve as good model for assessment of the effect of the anionic amino acid type on mineralization within the context of the beta-sheet structure. Mono layers of the peptides were deposited over simulated body solution (SBF1.5), and the effect of the adsorbing minerals over time was detected by Langmuir isotherm measurements, grazing incidence X-ray diffraction (GIXD) and Brewster angle microscopy (BAM). The results provide insight into mineralized film morphology and peptide lattice behavior during mineralization. The rigidity of the peptide template, along with the type of amino acid side chain, were found to significantly affect mineralization morphology and peptide structure. The results will contribute to a better understanding of calcium-phosphate mineralization in nature and in the context of biomaterials for applications in bone tissue regeneration.