Metabolomic Analysis Revealed Differential Adaptation to Salinity and Alkalinity Stress in Kentucky Bluegrass (Poa pratensis)
Metabolomic Analysis Revealed Differential Adaptation to Salinity and Alkalinity Stress in Kentucky Bluegrass (Poa pratensis)
复制标题
代谢组学分析揭示了早熟禾 (Poa pratensis) 对盐度和碱度胁迫的差异适应
DOI：
10.1007/s11105-014-0722-4
复制
发表时间：
2015-02-01
影响因子：
2.1
通讯作者：
Fu, Jinmin
中科院分区：
文献类型：
--
作者：
Hu, Longxing;Zhang, Pingping;Fu, Jinmin
植物可以通过不同的代谢反应在盐度和碱度下生存。本研究的目的是进一步了解代谢水平上对盐度和碱度胁迫的差异反应。两种肯塔基早熟禾“Midnight”（耐受）和“Voyager II”（敏感）在水培中接受盐度（NaCl，75 mM Na+，pH 5.41）和碱度（Na2CO3 和 NaHCO3，75 mM Na+，pH 9.5）的培养。使用气相色谱-质谱法（GC-MS）测定叶子代谢物谱。从垂直芽生长率（VSGR）、草坪质量、电解质渗漏（EL）和脂质过氧化（MDA 含量）来看，碱性盐比中性盐造成更严重的损害。植物对盐分的适应主要与氨基酸（脯氨酸、缬氨酸、谷氨酸、天冬酰胺、谷氨酰胺、苯丙氨酸和赖氨酸）和糖（蔗糖、海藻糖）的积累有关。相反，对碱度胁迫的代谢反应主要与有机酸的积累有关，有机酸主要是糖酸（葡萄糖酸、半乳糖二酸、葡萄糖二酸）和参与柠檬酸循环的有机酸（苹果酸、柠檬酸、异柠檬酸、琥珀酸、马来酸、乌头酸）。氨基酸、有机酸、糖和脂肪酸的差异积累可能有助于差异的适应策略，因为它们在应激反应途径中发挥作用，例如渗透调节、膜稳定性、能量产生的呼吸、碳/氮同化、细胞内pH值和根外pH值的调节。
Plants may survive salinity and alkalinity with differential metabolic responses. The aim of this study was to gain further insights into the differential response to salinity and alkalinity stress at the metabolic level. Two Kentucky bluegrass, 'Midnight' (tolerant) and 'Voyager II' (sensitive), were subjected to salinity (NaCl, 75 mM Na+, pH 5.41) and alkalinity (Na2CO3 and NaHCO3, 75 mM Na+, pH 9.5) in hydroponics. The leaf metabolite profile was determined using gas chromatography-mass spectrometry (GC-MS). Alkaline salts caused more severe damages than neutral salt as indicated by the vertical shoot growth rate (VSGR), turf quality, electrolyte leakage (EL) and lipid peroxidation (MDA content). Adaptation of plants to salinity was mainly associated with the accumulation of amino acids (proline, valine, glutamate, asparagine, glutamine, phenylalanine and lysine) and sugars (sucrose, trehalose). In contrast, the metabolic response to alkalinity stress was mostly associated with the accumulation of organic acids that are mainly sugar acids (gluconate, galactarate, glucarate) and those involved in the citric acid cycle (malate, citrate, isocitrate, succinate, maleiate, aconitate). The differential accumulation of the amino acids, organic acids, sugars and fatty acids may contribute to the differential adaptation strategies due to their roles in the stress response pathways such as osmotic adjustment, membrane stability, respiration for energy production, carbon/nitrogen assimilation, regulation of the intracellular pH and the pH outside roots.