Enzyme-linked immunosorbent assay for detection of Salmonella lipopolysaccharide in poultry specimens
Enzyme-linked immunosorbent assay for detection of Salmonella lipopolysaccharide in poultry specimens
复制标题
DOI：
10.1128/aem.47.6.1327-1330.1984
复制
发表时间：
1984-06
影响因子：
4.4
通讯作者：
C. Rigby
中科院分区：
文献类型：
--
作者：
C. Rigby
开发了一种用于检测沙门氏菌的酶联免疫吸附试验（ELISA），并通过使用人工污染的家禽饲料、粪便、垃圾或胴体冲洗标本以及自然污染的水样来评估。含有B或C2组沙门氏菌的标本分别抑制鼠伤寒沙门菌（B组）和奥尔巴尼沙门菌（C2组）脂多糖包被的微滴孔与多价抗o血清的结合。用Rhozyme 41（一种蛋白酶）处理标本可抑制非特异性反应。ELISA检测出111份培养阳性标本中有106份感染了血清B或C2型沙门氏菌。20份含C1组沙门氏菌标本中19份和36份培养阴性标本均为ELISA阴性。所有含有血清组B或C2沙门氏菌的7个水样，包括3个延迟二次富集后才培养阳性的水样，均为ELISA阳性。含有其他血清群沙门氏菌的9个水样中有7个和所有38个培养阴性样品均为ELISA阴性。ELISA操作简单，在48 h内产生结果，比培养法更经济。
An enzyme-linked immunosorbent assay (ELISA) for detection of salmonellae was developed and evaluated by using artificially contaminated specimens of poultry feed, feces, litter, or carcass rinsings, and naturally contaminated water samples. Specimens containing salmonellae of serogroups B or C2 inhibited the binding of polyvalent anti-O serum to microtiter plate wells coated with lipopolysaccharide of Salmonella typhimurium (serogroup B) or Salmonella albany (serogroup C2), respectively. Treatment of specimens with Rhozyme 41 (a protease) inhibited nonspecific reactions. The ELISA detected 106 of 111 culture-positive specimens contaminated with salmonellae of serogroups B or C2. Nineteen of 20 specimens containing salmonellae of serogroup C1 and all of 36 culture-negative specimens were ELISA negative. All seven water samples that contained salmonellae of serogroups B or C2, including three that were culture positive only after delayed secondary enrichment, were ELISA positive. Seven of the nine water samples that contained salmonellae of other serogroups, and all 38 culture-negative samples, were ELISA negative. The ELISA was simple to perform, produced results in 48 h, and was more economical than culture methods.