Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 9.
Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 9.
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DOI：
10.1107/s1744309109021460
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发表时间：
2009-07
期刊：
影响因子：
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通讯作者：
M. Mitchell;H. Nam;Adam Carter;Angela L McCall;Chelsea Rence;A. Bennett;Brittney L. Gurda;R. McKenna;Mark Porter;Y. Sakai;B. Byrne;N. Muzyczka;G. Aslanidi;S. Zolotukhin;M. Agbandje-McKenna
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文献类型：
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作者：
M. Mitchell;H. Nam;Adam Carter;Angela L McCall;Chelsea Rence;A. Bennett;Brittney L. Gurda;R. McKenna;Mark Porter;Y. Sakai;B. Byrne;N. Muzyczka;G. Aslanidi;S. Zolotukhin;M. Agbandje-McKenna
腺相关病毒(AAV)血清型9正在开发中，用于基因传递应用，与其他AAV血清型相比，它在肌肉中显示出显著增强的衣壳相关转导效率。为了研究AAV9病毒衣壳的结构决定因素，本文报道了AAV9病毒衣壳的纯化、结晶和初步的X射线结晶学分析。晶胞参数为a=b=251.0，c=640.0 A，晶胞内有三个完整的病毒衣壳。用分子置换的方法已经确定了不对称单元衣壳的取向和位置，结构测定正在进行中。
Adeno-associated virus (AAV) serotype 9, which is under development for gene-delivery applications, shows significantly enhanced capsid-associated transduction efficiency in muscle compared with other AAV serotypes. With the aim of characterizing the structural determinants of this property, the purification, crystallization and preliminary X-ray crystallographic analyses of the AAV9 viral capsid are reported. The crystals diffracted X-rays to 2.8 A resolution using synchrotron radiation and belonged to the trigonal space group P3(2), with unit-cell parameters a = b = 251.0, c = 640.0 A. There are three complete viral capsids in the crystal unit cell. The orientation and position of the asymmetric unit capsid have been determined by molecular-replacement methods and structure determination is in progress.