RAT RETINAL MULLER CELLS EXPRESS THY-1 FOLLOWING NEURONAL CELL-DEATH
RAT RETINAL MULLER CELLS EXPRESS THY-1 FOLLOWING NEURONAL CELL-DEATH
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DOI：
10.1002/glia.440140105
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发表时间：
1995-05-01
期刊：
影响因子：
6.2
通讯作者：
BARNSTABLE, CJ
中科院分区：
文献类型：
--
作者：
DABIN, I;BARNSTABLE, CJ
在正常大鼠视网膜中，Thy-1抗原是神经节细胞的特异性标志物，但体内神经节细胞变性并不能完全去除视网膜中Thy-1的表达。为了调和这些差异，我们假设神经节细胞死亡可以诱导神经胶质反应，包括Thy-1在Muller细胞（视网膜中的主要神经胶质细胞类型）中的表达。使用免疫细胞化学，我们已经表明，穆勒细胞的纯培养物强烈标记的抗体对Thy-1。从Muller细胞RNA逆转录的cDNA的PCR扩增表明Thy-1转录物的存在。用抗Thy 1和抗谷氨酰胺合成酶（Muller细胞的标记物）进行的双标记实验表明，在同一细胞中存在两种抗原。虽然穆勒细胞表达Thy-1 mRNA和蛋白质的情况下培养的神经元细胞，当与视网膜神经元共培养时，他们没有标记的抗体对Thy-1。我们的研究结果表明，Thy-1表达的Muller细胞后，视网膜神经元的损失。Thy-1可能在视网膜神经元死亡后的胶质反应中起重要作用。(C)1995年Wiley-Liss，Inc.
In the normal rat retina the Thy-1 antigen is a specific marker of ganglion cells, but degeneration of ganglion cells in vivo does not remove completely the expression of Thy-1 in the retina. To reconcile these differences we have postulated that ganglion cell death could induce a glial response including the expression of Thy-1 in Muller cells, the main glial cell type in the retina. Using immunocytochemistry, we have shown that pure cultures of Muller cells were strongly labelled with antibodies against Thy-1. PCR amplification of cDNA reverse transcribed from Muller cell RNA indicated the presence of Thy-1 transcripts. Double labelling experiments with anti-Thy1 and anti-glutamine synthetase, a marker of Muller cells, indicated the presence of both antigens in the same cells. Although Muller cells expressed Thy-1 mRNA and protein when cultured in the absence of neuronal cells, when co-cultured with retinal neurons they were not labelled with antibodies against Thy-1. Our results suggest that Thy-1 is expressed by Muller cells following loss of retinal neurons. Thy-1 may have an important function during glial response to neuron death in retina. (C) 1995 Wiley-Liss, Inc.