Characterization of novel staphylococcal enterotoxin-like toxin type P
Characterization of novel staphylococcal enterotoxin-like toxin type P
复制标题
DOI：
10.1128/iai.73.9.5540-5546.2005
复制
发表时间：
2005-09-01
影响因子：
3.1
通讯作者：
Shinagawa, K
中科院分区：
文献类型：
--
作者：
Omoe, K;Imanishi, K;Shinagawa, K
我们研究了一种新的葡萄球菌肠毒素（SE）样毒素P型（SEIP）的生物学特性。当以0.4pM（0.01ng/ml）或更高的浓度施用时，SEIP诱导大量增殖应答和细胞因子白细胞介素-2、γ干扰素、肿瘤坏死因子α和白细胞介素-4从人T细胞的产生。辅助细胞上主要组织相容性复合物II类分子的表达是SEIP刺激T细胞所必需的。SEIP选择性刺激大量携带受体V β 5.1、6、8、16、18和21.3的人T细胞。这些结果表明，SEIP作为超抗原。SEIP在家麝鼩呕吐试验中证明具有呕吐作用，尽管剂量相对较高（50至150 μ g/动物）。用30株携带selp的金黄色葡萄球菌进行的SEIP生产的定量测定显示，这些菌株中的60%在体外产生显著量的SEIP。所有10株携带seb和selp的菌株均产生SEB，但不产生SEIP，表明在S.金黄色葡萄球菌菌株具有特定的SE基因组成。
We investigated the biological properties of a novel staphylococcal enterotoxin (SE)-like toxin type P (SEIP). SEIP induced a substantial proliferative response and the production of cytokines interleukin-2, gamma interferon, tumor necrosis factor alpha, and interleukin-4 from human T cells when administered at a concentration of 0.4 pM (0.01 ng/ml) or more. The expression of major histocompatibility complex class II molecules on accessory cells was required for T-cell stimulation by SEIP. SEIP selectively stimulated a vast number of human T cells bearing receptors V beta 5.1, 6, 8, 16, 18, and 21.3. These results indicated that SEIP acts as a superantigen. SEIP proved to be emetic in the house musk shrew emetic assay, although at a relatively high dose (50 to 150 tLg/animal). A quantitative assay of SEIP production with 30 Staphylococcus aureus strains harboring selp showed that 60% of these strains produced significant amounts of SEIP in vitro. All 10 strains carrying seb and selp produced SEB but not SEIP, suggesting the inactivation of the selp locus in S. aureus strains with a particular se gene constitution.