The RNA virome of echinoderms
The RNA virome of echinoderms
复制标题
棘皮动物的 RNA 病毒组
DOI：
10.1099/jgv.0.001772
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发表时间：
2022
影响因子：
3.8
通讯作者：
Hewson, Ian
中科院分区：
文献类型：
--
作者：
Jackson, Elliot W.;Wilhelm, Roland C.;Buckley, Daniel H.;Hewson, Ian
棘皮动物是海洋无脊椎动物的一个门，包括模式生物、关键物种和商业捕捞的海鲜动物。尽管感染棘皮动物的 RNA 病毒具有科学、生态和经济重要性，但与其他无脊椎动物相比，人们对它们的多样性知之甚少。我们筛选了 900 多个转录组和病毒宏基因组，以表征来自所有五个纲（海百合总科、海参总科、小星总科、蛇形总科和棘皮总科）的 38 种棘皮动物物种的 RNA 病毒组。我们鉴定了 347 个病毒基因组片段，这些片段被分类为九个病毒目中的属和科：小核病毒目、Durnavirales、Martellivirales、Nodamuvirales、Reovirales、Amarillovirales、Ghabrivirales、Mononegavirales 和 Hepelivirales。我们比较了三个生命阶段（胚胎、幼虫、成虫）的相对病毒表现，并表征了编码完整或接近完整基因组的重叠群的基因内容。尽管大多数病毒重叠群是从成体组织的转录组中发现的，但未发现给定转录组中病毒读数的比例在生命阶段之间存在显着差异。这项研究阐明了棘皮动物 RNA 病毒的生物多样性，揭示了自然群体中病毒群的出现。
Echinoderms are a phylum of marine invertebrates that include model organisms, keystone species, and animals commercially harvested for seafood. Despite their scientific, ecological, and economic importance, there is little known about the diversity of RNA viruses that infect echinoderms compared to other invertebrates. We screened over 900 transcriptomes and viral metagenomes to characterize the RNA virome of 38 echinoderm species from all five classes (Crinoidea, Holothuroidea, Asteroidea, Ophiuroidea and Echinoidea). We identified 347 viral genome fragments that were classified to genera and families within nine viral orders -Picornavirales, Durnavirales, Martellivirales, Nodamuvirales, Reovirales, Amarillovirales, Ghabrivirales, Mononegavirales,andHepelivirales. We compared the relative viral representation across three life stages (embryo, larvae, adult) and characterized the gene content of contigs which encoded complete or near-complete genomes. The proportion of viral reads in a given transcriptome was not found to significantly differ between life stages though the majority of viral contigs were discovered from transcriptomes of adult tissue. This study illuminates the biodiversity of RNA viruses from echinoderms, revealing the occurrence of viral groups in natural populations.