Changing Metabolism in Differentiating Cardiac Progenitor Cells-Can Stem Cells Become Metabolically Flexible Cardiomyocytes?
Changing Metabolism in Differentiating Cardiac Progenitor Cells-Can Stem Cells Become Metabolically Flexible Cardiomyocytes?
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DOI：
10.3389/fcvm.2018.00119
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发表时间：
2018
影响因子：
3.6
通讯作者：
Carr CA
中科院分区：
文献类型：
--
作者：
Malandraki-Miller S;Lopez CA;Al-Siddiqi H;Carr CA
心脏是代谢杂食动物，成年心脏选择最适合每种情况的底物，优选脂肪酸氧化以满足收缩心肌的高能量需求。胎儿心脏存在于缺氧环境中，并通过糖酵解获得大部分能量。出生后，葡萄糖和脂肪酸氧化代谢的"胎儿开关"与再生表型的丧失有关。各种类型的干细胞已被用于分化研究，但大多数是在高葡萄糖培养基中培养。这在大多数心脏分化方案中没有改变。尽管代谢状态影响标志物表达和细胞功能和活性，但底物组成目前被忽视。在这篇综述中，我们讨论了心脏代谢在发展过程中的变化，用于祖细胞分化为心肌细胞的各种协议，什么是已知的干细胞代谢和代谢的考虑如何有助于干细胞衍生的心肌细胞的成熟。
The heart is a metabolic omnivore and the adult heart selects the substrate best suited for each circumstance, with fatty acid oxidation preferred in order to fulfill the high energy demand of the contracting myocardium. The fetal heart exists in an hypoxic environment and obtains the bulk of its energy via glycolysis. After birth, the “fetal switch” to oxidative metabolism of glucose and fatty acids has been linked to the loss of the regenerative phenotype. Various stem cell types have been used in differentiation studies, but most are cultured in high glucose media. This does not change in the majority of cardiac differentiation protocols. Despite the fact that metabolic state affects marker expression and cellular function and activity, the substrate composition is currently being overlooked. In this review we discuss changes in cardiac metabolism during development, the various protocols used to differentiate progenitor cells to cardiomyocytes, what is known about stem cell metabolism and how consideration of metabolism can contribute toward maturation of stem cell-derived cardiomyocytes.