Characterization of the genome of the agent of erythrocyte aplasia permits its classification as a human parvovirus.
Characterization of the genome of the agent of erythrocyte aplasia permits its classification as a human parvovirus.
复制标题
红细胞再生障碍因子的基因组表征允许将其分类为人类细小病毒。
DOI：
10.1099/0022-1317-64-11-2527
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Anderson,MJ
中科院分区：
文献类型：
--
作者：
Summers,J;Jones,SE;Anderson,MJ
最近已经确定，病毒感染是骨髓中红细胞生成短暂停滞的最常见原因，导致慢性溶血性贫血患者的再生障碍性危象。这种人类病毒的物理特征表明它可能是细小病毒科的一员。我们在这里报告，在退火条件下从该病毒中提取的核酸产生了一个单一的物种的双链DNA的长度为5.5 kb。用热或碱处理将这种DNA转化为对单链特异性核酸酶S1敏感的快速迁移形式。在预期不会发生退火的低离子强度条件下提取病毒体DNA，得到与5.5kb单链分子共迁移的DNA。结果表明，该病毒将相同数量的互补DNA链包装成单独的病毒体。这表明，该病毒可以被归类为细小病毒属的成员。
It has recently been established that infection with a virus is the most common cause of transient arrest of erythrocyte production in the bone marrow, leading to aplastic crisis, in persons suffering chronic haemolytic anaemias. The physical characteristics of this human virus have suggested that it may be a member of theParvoviridae. We report here that extraction of nucleic acid from this virus under annealing conditions yielded a single species of double-stranded DNA 5.5 kb in length. Treatment with heat or alkali converted this DNA into a rapidly migrating form sensitive to the single-strand-specific nuclease S1. Extraction of the virion DNA under conditions of low ionic strength where annealing would not be expected to occur yielded DNA which comigrated with the 5.5 kb single-stranded molecule. The results indicate that this virus packages equal numbers of complementary DNA strands into separate virions. It is suggested that this virus can be classified as a member of the genus parvovirus.