Molecular Response of Rabbit Menisci to Surgically Induced Hemarthrosis and a Single Intra-Articular Dexamethasone Treatment
Molecular Response of Rabbit Menisci to Surgically Induced Hemarthrosis and a Single Intra-Articular Dexamethasone Treatment
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DOI：
10.1002/jor.24346
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发表时间：
2019-09-01
影响因子：
2.8
通讯作者：
Hart, David A.
中科院分区：
文献类型：
--
作者：
Heard, Bryan J.;Barton, Kristen I.;Hart, David A.
前交叉韧带重建手术可以恢复生物力学稳定性，但这种手术不能可靠地预防创伤后骨关节炎的发生。本研究的目的是阐明手术损伤后关节间隙出血的分子反应，然后研究地塞米松（DEX）对这种分子反应的影响。还进行了急性受控暴露于血液和血液加DEX后的细胞活力研究。将48只新西兰白色家兔随机分为对照组、假手术组、手术组和手术+ DEX组（每组n = 6）。在48 h和9周时处死动物，并收获脑组织。对关键炎症和降解蛋白的信使RNA（mRNA）表达水平以及自噬途径分子的mRNA水平进行定量，并描述了统计学显著性变化。通过在自体血液或自体血液加DEX中孵育内侧和外侧半月板组48小时（每组n = 4;总共8个内侧半月板和8个外侧半月板），然后进行组织学活/死测定来计算半月板细胞活力。结果表明，当组织暴露于与DEX组合的血液时，仅中膜细胞活力显著降低。在手术后48小时和9周，手术后单次给予DEX显著抑制了椎间盘内关键炎症和降解标志物的分子表达升高。在体外，自体血不影响细胞活力，但DEX的加入独特地影响了中膜。(c)2019骨科研究学会。由威利期刊公司出版J Orthop Res 37：2043-2052，2019
Anterior cruciate ligament reconstructive surgery can restore biomechanical stability, however, such surgery cannot reliably prevent the onset of post-traumatic osteoarthritis. The aim of this study was to elucidate the molecular response that occurs within the menisci following a surgical injury that allows bleeding into the joint space, and then to investigate the effect of dexamethasone (DEX) on this molecular response. Cell viability studies following acute controlled exposure to blood and blood plus DEX were also conducted. Forty-eight New Zealand white rabbits were randomly allocated into control, sham, surgical, and surgical + DEX groups (each group n = 6). Animals were sacrificed at 48 h and 9 weeks, and menisci were harvested. The messenger RNA (mRNA) expression levels for key inflammatory, and degradative proteins, as well as mRNA levels for autophagy pathway molecules were quantified, and statistically significant changes were described. Meniscal cell viability was calculated by incubating groups of medial and lateral menisci in autologous blood, or autologous blood plus DEX for 48 h (each group n = 4; total of eight medial and eight lateral menisci), and then conducting a histological live/dead assay. Results indicated a significant reduction in only medial meniscal cell viability when the tissue was exposed to blood in combination with DEX. A single administration of DEX following surgery significantly suppresses the elevated molecular expression for key inflammatory and degradative markers within menisci at 48 h and 9 weeks post-surgery. In vitro, autologous blood did not affect cell viability, but addition of DEX uniquely impacted the medial menisci. (c) 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2043-2052, 2019