Differential in vivo internalization of MOR-1 and MOR-1C by morphine
Differential in vivo internalization of MOR-1 and MOR-1C by morphine
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DOI：
10.1097/00001756-200110080-00017
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发表时间：
2001-10-08
期刊：
影响因子：
1.7
通讯作者：
Pasternak, GW
中科院分区：
文献类型：
--
作者：
Abbadie, C;Pasternak, GW
μ阿片受体莫尔-1被许多μ激动剂内化，但不是吗啡。为了了解细胞内羧基末端的差异是否影响内化，我们研究了小鼠体内侧隔中μ阿片受体莫尔-1C剪接变体的内化。脑室内（i. c. v.）生理盐水处理后，未处理小鼠神经元内的莫尔-1C样免疫反应性（L1）主要在靠近质膜的簇中发现。脑室注射[D-Ala（2），MePhe（4），Gly（ol）（5）]脑啡肽（DAMGO）或吗啡后，莫尔-1C-L1在胞浆内聚集成内体。这种作用被阿片类拮抗剂纳洛酮的预先给药抑制。相反，只有DAMGO而不是吗啡内化莫尔-1-L1。这些结果说明了在COOH末端具有选择性剪接的两种莫尔-1变体之间内在化的差异。NeuroReport 12：3069-3072（C）2001 Lippincott威廉姆斯和威尔金斯。
The mu opioid receptor MOR-1 is internalized by many mu agonists, but not morphine. To see whether differences in the intracellular carboxy terminus influences internalization, we examined internalization of a splice variant of the mu opioid receptor, MOR-1C, in the lateral septum of the mouse in vivo. Following intracerebroventricular (i.c.v.) saline treatment, MOR-1C-like immunoreactivity (L1) within neurons in naive mice was found predominantly in clusters close to the plasma membrane. Following either intracerebroventricular [D-Ala(2),MePhe(4),Gly(ol)(5)]enkephalin (DAMGO) or morphine, MOR-1C-L1 clustered into endosomes in the cytoplasm. This effect was suppressed by prior administration of the opioid antagonist naloxone. In contrast, only DAMGO, and not morphine, internalized MOR-1-L1. These results illustrate differences in internalization between two MOR-1 variants that have alternative splicing at the COOH terminus. NeuroReport 12:3069-3072 (C) 2001 Lippincott Williams & Wilkins.