Functional analysis of the CXCR1a gene response to SGIV viral infection in grouper
Functional analysis of the CXCR1a gene response to SGIV viral infection in grouper
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石斑鱼 CXCR1a 基因对 SGIV 病毒感染反应的功能分析
DOI：
10.1016/j.fsi.2019.02.046
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发表时间：
2019
影响因子：
4.7
通讯作者：
Qin Qiwei
中科院分区：
文献类型：
--
作者：
Wang Qing;Yang Min;Li Chen;Wang Shaowen;Wang Yuxin;Lin Fangmei;Zheng Leyun;Yu Yepin;Qin Qiwei
趋化因子受体是七跨膜域 G 偶联受体的超家族，在免疫监视、炎症和发育中发挥重要作用。在之前的研究中，在石斑鱼（Epinephelus coioides）中鉴定出了一系列CXCR；然而，CXCR在病毒感染中的功能尚未被研究。为了更好地了解CXCR家族对鱼类免疫反应的影响，克隆了全长CXCR1a，并研究了其对新加坡石斑鱼虹彩病毒（SGIV）的免疫反应。石斑鱼 CXCR1a 共享一个七次跨膜 (7-TM) 区域和一个包含 triaa 延伸（DRY 基序）的 G 蛋白偶联受体 (GPCR) 家族 1。系统发育分析表明，CXCR1a 与红鳍东方鲀的亲缘关系最近，其次是其他鱼类、鸟类和哺乳动物物种。荧光显微镜显示CXCR1a主要在细胞质中表达。石斑鱼细胞中CXCR1a的过度表达显着抑制SGIV的复制，表明CXCR1a延迟了SGIV感染诱导的细胞病变效应（CPE）的发生并抑制病毒基因转录。此外，我们的结果还表明，CXCR1a 过表达显着增加了干扰素相关细胞因子的表达，并激活了 ISRE 和 IFN 启动子活性。综上所述，结果表明CXCR1a可能具有针对SGIV感染的抗病毒功能。
Chemokine receptors are a superfamily of seven-transmembrane domain G-coupled receptors and have important roles in immune surveillance, inflammation, and development. In previous studies, a series of CXCRs in grouper (Epinephelus coioides) was identified; however, the function of CXCR in viral infection has not been studied. To better understand the effect of the CXCR family on the fish immune response, full-length CXCR1a was cloned, and its immune response to Singapore grouper iridovirus (SGIV) was investigated. Grouper CXCR1a shared a seven-transmembrane (7-TM) region and a G protein-coupled receptor (GPCR) family 1 that contained a triaa stretch (DRY motif). Phylogenetic analysis indicated that CXCR1a showed the nearest relationship to Takifugu rubripes, followed by other fish, bird and mammal species. Fluorescence microscopy revealed that CXCR1a was expressed predominantly in the cytoplasm. Overexpression of CXCR1a in grouper cells significantly inhibited the replication of SGIV, demonstrating that CXCR1a delayed the occurrence of cytopathic effects (CPE) induced by SGIV infection and inhibited viral gene transcription. Furthermore, our results also showed that CXCR1a overexpression significantly increased the expression of interferon-related cytokines and activated ISRE and IFN promoter activities. Taken together, the results demonstrated that CXCR1a might have an antiviral function against SGIV infection.