Immune trypanolysis test with blood spotted on filter paper for epidemiological surveillance of sleeping sickness
Immune trypanolysis test with blood spotted on filter paper for epidemiological surveillance of sleeping sickness
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DOI：
10.1111/tmi.12316
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发表时间：
2014-07-01
影响因子：
3.3
通讯作者：
Jamonneau, Vincent
中科院分区：
文献类型：
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作者：
Camara, Oumou;Camara, Mamadou;Jamonneau, Vincent
免疫锥虫溶解试验（TL）是一种准确的血清诊断工具，越来越多地用于昏睡病的医学监测，但它仅限于参考实验室。为了便于测试样本的储存和运输，我们开发了一种用于检查滤纸（TL-fp）上的血液斑点的方案，该方案可以在环境温度下储存和运输。我们比较了它的性能与经典的TL血浆（TL-PL），需要保持冷冻，直到use.METHODS的研究进行了活动的几内亚共和国的焦点。结果TL-fp法检测的阳性率明显低于TL-pl法，而确诊的昏睡病患者TL-fp检测均为阳性。fp似乎在可行性和灵敏度之间提供了一个很好的折衷，以检测在传播布氏冈比亚锥虫，并可用于促进冈比亚昏睡病的消除。
OBJECTIVES The immune trypanolysis test (TL) is an accurate sero-diagnostic tool increasingly implemented for sleeping sickness medical surveillance, but it is restricted to the reference laboratories. To facilitate storage and transport of the test specimen, we developed a protocol for the examination of blood spotted on filter paper (TL-fp) that can be stored and shipped at ambient temperature. We compared its performance with the classical TL on plasma (TL-pl) that needs to be kept frozen until use.METHODS The study was conducted in active foci of the Republic of Guinea. In total, 438 specimens from treated and untreated sleeping sickness patients and serological suspects were tested with both methods.RESULT TL-fp gave significantly less positive results than TL-pl, but all the confirmed sleeping sickness cases were positive with the TL-fp protocol.CONCLUSION TL-fp appears to offer a good compromise between feasibility and sensitivity to detect currently infected subjects who play a role in the transmission of Trypanosoma brucei gambiense and is useful for contributing to the elimination of gambiense sleeping sickness.