Characteristics of Mesenchymal Stromal Precursor Cells Labeled with Lentiviral Vector in Long-Term Bone Marrow Culture
Characteristics of Mesenchymal Stromal Precursor Cells Labeled with Lentiviral Vector in Long-Term Bone Marrow Culture
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慢病毒载体标记的间充质基质前体细胞在长期骨髓培养中的特性
DOI：
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发表时间：
2010
影响因子：
0.7
通讯作者：
N. Drize
中科院分区：
文献类型：
--
作者：
N. Sats;I. Shipounova;A. Bigildeev;D. Svinareva;O. Zhironkina;N. Drize
用慢病毒载体标记小鼠骨髓间充质前体细胞。我们研究了标记细胞在长期骨髓培养基质亚层和由标记细胞形成的异位造血灶中的命运。我们还分析了标记多能成纤维细胞CFU在同基因小鼠肾包膜下长期骨髓培养亚层和由这些亚层形成的异位造血灶中的发生率。结果表明，标记基因存在于约40%的间质亚层细胞和30%的成纤维细胞CFU中，有效的基因转移不影响造血细胞的总产量。肾包膜下长期骨髓培养标记亚层植入后形成的异位造血灶大小与对照组无差异。在这些病灶中，40%的骨壳分化细胞携带标记基因。对这些病灶中成纤维细胞CFU的分析显示，尽管成纤维细胞CFU的总浓度与骨髓中相当，但标记成纤维细胞CFU的浓度约为6%，这表明在间充质干细胞和成纤维细胞CFU之间的基质细胞层次中可能存在另一类前体。我们的研究结果证明了间充质干细胞在不丢失整合到基因组中的标记基因的情况下自我维持和分化的能力。
Mouse mesenchymal stromal precursor cells were labeled with lentiviral vector in long-term bone marrow culture. We studied the fate of labeled cells in the stromal sublayer of the longterm bone marrow culture and in ectopic hemopoiesis foci formed from the labeled cultures. The incidence of labeled polypotent fibroblast CFU in sublayers of long-term bone marrow culture and in ectopic hemopoiesis foci formed from these sublayers under the renal capsule of syngeneic mice was also analyzed. It was shown that the marker gene was present in about 40% cells of the stromal sublayer and 30% fibroblast CFU and that effective gene transfer did not affect the total production of hemopoietic cells. The size of ectopic hemopoietic foci formed after implantation of labeled sublayers of the long-term bone marrow culture under the renal capsule did not differ from the control. Differentiated cells of the osseous shell in these foci carried the marker gene in 40% cases. Analysis of fibroblast CFU in these foci showed that despite the total concentration of fibroblast CFU was comparable to that in the bone marrow, the concentration of labeled fibroblast CFU was about 6%, which suggests that one more class of precursors probably exists in the hierarchy of stromal cells presumably between mesenchymal stem cells and fibroblast CFU. Our findings demonstrate the capacities of mesenchymal stem cells to self-maintenance and differentiation without loosing the marker gene integrated into the genome.
影响因子：
2.6
作者：
Lee, C. Chang I.;Christensen, Jared E.;Yoder, Mervin C.;Tarantal, Alice F.
通讯作者：
Tarantal, Alice F.