Expression of multidrug resistance-associated protein 2 in small intestine from pregnant and postpartum rats
Expression of multidrug resistance-associated protein 2 in small intestine from pregnant and postpartum rats
复制标题
DOI：
10.1152/ajpgi.2001.280.6.g1261
复制
发表时间：
2001-06-01
影响因子：
4.5
通讯作者：
Vore, M
中科院分区：
文献类型：
--
作者：
Mottino, AD;Hoffman, T;Vore, M
We analyzed the expression of multidrug resistance-associated protein 2 (mrp2) in the small intestine of control female rats and in rats during late pregnancy (19-20 days of pregnancy) and lactation (2-4, 10-14, and 21 days after delivery). Western blot analysis was performed on brush-border membranes prepared from different regions of the small intestine. Expression of mrp2 was maximal in the proximal segments for all experimental groups, was preserved in pregnant rats, and increased by 100% in postpartum rats by late lactation with respect to control animals. Northern blot analysis of mrp2 mRNA revealed a positive correlation with protein levels. Transport of S-glutathione-dinitrophenol (DNP-SG) from the intestinal cell to the lumen was analyzed in the everted intestinal sac model. Secretion of DNP-SG was not altered in pregnant rats but increased in lactating animals by late lactation. Intestinal mrp2 mRNA, protein, and transport activity are increased in lactating rats, suggesting that this may represent an adaptive mechanism to minimize the toxicity of dietary xenobiotics in response to increased postpartum food consumption.
