Generation of a cytotoxic T‐lymphocyte response using a Salmonella antigen‐delivery system
Generation of a cytotoxic T‐lymphocyte response using a Salmonella antigen‐delivery system
复制标题
使用沙门氏菌抗原递送系统产生细胞毒性 T 淋巴细胞反应
作者：
J. Flynn;W. Weiss;K. Norris;H. Seifert;S. Kumar;M. So
我们构建了一种通用载体，用于在减毒鼠伤寒沙门氏菌染色体上克隆和稳定表达外源基因。使用这种染色体表达载体（CEV），我们在 aroA 鼠伤寒沙门氏菌菌株中表达了小鼠疟疾约氏疟原虫的环子孢子（CS）基因。用表达 CS 的沙门氏菌重组体免疫的小鼠会产生 CS 特异性细胞毒性 T 淋巴细胞 (CTL) 反应。这是首次证明减毒沙门氏菌可以在小鼠体内引发对外源蛋白的特异性 CTL 反应。轻松稳定地表达沙门氏菌染色体外源基因以及生成特异性 CTL 的能力极大地扩展了沙门氏菌作为抗原递送系统的潜力。
We have constructed a general‐use vector for the cloning and stable expression of foreign genes in the chromosome of attenuated Salmonella typhimurium. Using this chromosomal expression vector (CEV), we expressed the circumsporozoite (CS) gene of the mouse malaria Plasmodium yoelii in an aroA S. typhimurium strain. Mice immunized with CS‐expressing Salmonella recombinants mount a CS‐specific cytotoxic T‐lymphocyte (CTL) response. This is the first demonstration that attenuated Salmonella can elicit a specific CTL response to a foreign protein in mice. The ability to easily and stably express foreign genes from the Salmonella chromosome and the generation of specific CTL greatly expands the potential of Salmonella as an antigen‐delivery system.
lacZ 翻译起始突变。
影响因子：
5.6
作者：
Munson,LM;Stormo,GD;Niece,RL;Reznikoff,WS
通讯作者：
Reznikoff,WS