Neuropeptide Y Y1 receptor-mediated biodegradable photoluminescent nanobubbles as ultrasound contrast agents for targeted breast cancer imaging
Neuropeptide Y Y1 receptor-mediated biodegradable photoluminescent nanobubbles as ultrasound contrast agents for targeted breast cancer imaging
复制标题
神经肽 Y Y-1 受体介导的可生物降解光致发光纳米气泡作为超声造影剂用于乳腺癌靶向成像
DOI：
10.1016/j.biomaterials.2016.11.028
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发表时间：
2017-02-01
期刊：
影响因子：
14
通讯作者：
Wu, Aiguo
中科院分区：
文献类型：
--
作者：
Li, Juan;Tian, Yuchen;Wu, Aiguo
靶向分子成像在肿瘤的诊断和治疗中引起了广泛的关注。然而，大多数临床使用的超声造影剂（UCAs）是非靶向微泡，很少用于癌症成像。在这里，我们将液体四氟己烷（C6F14）包封在生物可降解的光致发光聚合物（BPLPs）中，通过乳液蒸发过程和PNBL-NPY配体的偶联制备荧光纳米泡（NBs），以特异性靶向乳腺肿瘤中过表达的Y-1受体。所制得的PNBL-NPY改性NBs尺寸均匀，具有良好的分散性和光稳定性，超声增强效果好。此外，体外和体内实验结果表明，制备的NBs对Y-1受体过表达的乳腺癌细胞和肿瘤具有高亲和力和特异性，毒性和器官损伤最小。我们开发的pnbl - npy修饰的NBs是一种安全、高效、特异性靶向乳腺癌成像的新型靶向uca，可能为未来早期癌症诊断和治疗提供新的纳米平台。(C) 2016 Elsevier Ltd.版权所有。
Targeted molecular imaging has attracted great attention in cancer diagnosis and treatment. However, most clinically used ultrasound contrast agents (UCAs) are non-targeted microbubbles seldom used for cancer imaging. Here, we fabricated fluorescent nanobubbles (NBs) by encapsulation of liquid tetrade-cafluorohexane (C6F14) within biodegradable photoluminescent polymers (BPLPs) through an emulsion evaporation process and conjugation of PNBL-NPY ligand for specific targeting of Y-1 receptors overexpressed in breast tumors. The developed PNBL-NPY modified NBs were uniform in size with good dispersibility and photostability, presenting good ultrasound enhancement. Further, in vitro and in vivo results indicated that the fabricated NBs exhibit high affinity and specificity to Y-1 receptor-overexpressing breast cancer cells and tumors with minimal toxicity and damage to organs. Our developed PNBL-NPY-modified NBs are novel targeted UCAs for safe, efficient and specific targeted breast cancer imaging, and may provide a new nanoplatform for early cancer diagnosis and treatment in the future. (C) 2016 Elsevier Ltd. All rights reserved.