[Effect and mechanism of total flavone of epimedium on primary callus formation in ovariectomized rats with fractures].
[Effect and mechanism of total flavone of epimedium on primary callus formation in ovariectomized rats with fractures].
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DOI：
10.3969/j.issn.1003-0034.2017.08.013
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发表时间：
2017-08-25
影响因子：
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通讯作者：
Li, Chun-Wen
中科院分区：
文献类型：
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作者：
Shi, Xiao-Lin;Liang, Bo-Cheng;Li, Chun-Wen
目的：探讨淫羊藿总黄酮（total flavone of epimedium treatment， TFE）对去卵巢大鼠初生愈伤组织形成的影响及其机制。方法：选取6 ~ 8周龄雄性SD大鼠40只，体重209 ~ 246 g。我们在卵巢切除术后6周建立一个股骨骨折伴骨干中段骨折的模型，估计并随机分为TFE组（150 mg·kg⁻）和对照组（接受生理盐水）。采用HE染色评价两组骨痂愈合过程中初代愈伤组织形态的差异。实时聚合酶链反应测定愈伤组织中runt相关转录因子2(Runx2) mRNA的相对表达量。采用免疫组化技术观察两组愈伤组织中酪蛋白激酶2-相互作用蛋白1（CKIP-1）蛋白水平。采用三点弯曲试验测试最大断裂载荷。结果：骨密度、初生愈伤组织体积、骨小梁成员(Tb；N)和小梁厚度(Tb。TFE组的Th均高于对照组(P
OBJECTIVE: To explore the effects and related mechanisms of total flavone of epimedium treatment(TFE)on primary callus for mation in ovariectomized rats.METHODS: Forty male SD rats weighted from 209 to 246 g and aged 6 to 8 weeks were selected. Six weeks after ovariectomy a femur fracture model with middiaphyseal segment fracture was established, estimated and randomly divided into TFE group (150 mg·kg⁻·d⁻) and control group(received saline). HE staining was used to evaluate the morphologic difference of primary callus during the bone callus healing between these two groups. The relative expression of Runt-related transcription factor 2(Runx2) mRNA in the callus was identified by real-time polymerase chain reaction. Immunohistochemical technique was used to observe the Casein kinase 2-interacting protein 1(CKIP-1) protein level in the callus of the two groups. Maximum fracture load was tested by three point bend test.RESULTS: The BMD, primary callus volume, trabecular member(Tb.N) and trabecular thickness(Tb.Th) were higher in TFE group than that in control group(P