The use of laryngeal mucosa mesenchymal stem cells for the repair the vocal fold injury
The use of laryngeal mucosa mesenchymal stem cells for the repair the vocal fold injury
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喉粘膜间充质干细胞修复声带损伤的应用
DOI：
10.1016/j.biomaterials.2013.08.004
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发表时间：
2013-12-01
期刊：
影响因子：
14
通讯作者：
Deng, Zhihong
中科院分区：
文献类型：
--
作者：
Peng, Han;Ming, Leiguo;Deng, Zhihong
干细胞移植是一种有吸引力的新方法，可以补充传统的修复或手术技术，用于再生受伤或病理性损伤的喉组织。然而，最佳的细胞递送策略仍有待确定。本研究的目的是建立损伤声带愈合的新策略，利用喉粘膜间充质干细胞分化为肌成纤维细胞或成纤维细胞，改善声带损伤重建微环境，作为喉组织工程种子细胞的新替代方案。分离和扩增后，细胞被鉴定为贴壁间充质细胞，在体外具有显着的增殖潜力，并通过流式细胞术进行表征。通过脂肪生成、骨生成和软骨细胞培养证实，间充质细胞的分化潜力在增殖过程中得以维持。当LM-MSC移植到损伤的声带后，具有强效分化为肌成纤维细胞和成纤维细胞的能力，可以调节细胞外基质，阻断胶原蛋白和纤连蛋白的快速增加，抑制弹力纤维和HA的快速减少，减轻微环境炎症反应，防止声带疤痕的形成。 (C) 2013 Elsevier Ltd. 保留所有权利。
Stem cell transplantation is a kind of attractive and new approach that complements traditional restorative or surgical techniques for the regeneration of injured or pathologically damaged laryngeal tissues. However, the best cell delivery strategy remains to be identified. The objective of this study was to establish a new strategy to the healing of injured vocal fold, using laryngeal mucosa mesenchymal stem cells differentiating into myofibroblasts or fibroblasts and improving the reconstruction microenvironment in the vocal fold injury as a new alternative as seed cells for laryngeal tissue engineering. After isolation and expansion, cells were identified as adherent mesenchymal cells with substantial proliferation potential in vitro, and were also characterized by flow cytometry. The differentiation potential of mesenchymal cells was maintained during proliferation as confirmed by culturing for adipogenesis, osteogenesis and chondrocyte. When LM-MSC was transplanted into the injured vocal fold, it has the potent differentiated into myofibroblasts and fibroblasts, which could regulate extracellular matrix, block collagen and the fibronectin rapid increased, inhibit the rapidly decrease of elastic fiber and HA, decrease the microenvironment inflammatory reaction, and prevent the formation of vocal fold scar. (C) 2013 Elsevier Ltd. All rights reserved.