Neuroendocrine effects of perfluorooctane sulfonate in rats.
Neuroendocrine effects of perfluorooctane sulfonate in rats.
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DOI：
10.1289/ehp.6128
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发表时间：
2003-09
影响因子：
10.4
通讯作者：
MohanKumar, Sheba M J
中科院分区：
文献类型：
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作者：
Austin, Maureen E;Kasturi, Badrinarayanan S;Barber, Matthew;Kannan, Kurunthachalam;MohanKumar, Puliyur S;MohanKumar, Sheba M J
全氟辛烷磺酸(PFOS)是磺基含氟化合物的降解产物，广泛应用于工业和家庭。人类和野生动物接触到这类化合物的来源有很多。全氟辛烷磺酸对啮齿动物的毒性测试引起了人们对全氟辛烷磺酸潜在的发育、生殖和全身影响的关注。然而，到目前为止，全氟辛烷磺酸对神经内分泌系统的影响还没有得到研究。在本研究中，成年雌性大鼠以0、1或10 mg/kg体重(BW)连续2周腹腔注射全氟辛烷磺酸(PFOS)。每天监测食物和水的摄入量、体重和发情周期。治疗结束后，采用高效液相色谱-电喷雾质谱联用技术检测组织中全氟辛烷磺酸水平。电化学法测定脑内单胺类物质的变化，放射免疫法测定血清皮质酮和瘦素水平。全氟辛烷磺酸治疗导致这种化学物质在包括大脑在内的各种身体组织中呈剂量依赖性积累。全氟辛烷磺酸暴露以剂量依赖的方式减少食物摄入量和体重。全氟辛烷磺酸治疗影响了动情周期，增加了血清皮质酮水平，同时降低了血清瘦素水平。全氟辛烷磺酸治疗还增加了下丘脑室旁核中去甲肾上腺素的浓度。这些结果表明，全氟辛烷磺酸暴露可影响大鼠的神经内分泌系统。
Perfluorooctane sulfonate (PFOS) is a degradation product of sulfonyl-based fluorochemicals that are used extensively in industrial and household applications. Humans and wildlife are exposed to this class of compounds from several sources. Toxicity tests in rodents have raised concerns about potential developmental, reproductive, and systemic effects of PFOS. However, the effect of PFOS on the neuroendocrine system has not been investigated thus far. In this study, adult female rats were injected intraperitoneally with 0, 1, or 10 mg PFOS/kg body weight (BW) for 2 weeks. Food and water intake, BW, and estrous cycles were monitored daily. At the end of treatment, PFOS levels in tissues were measured by high-performance liquid chromatography (HPLC) interfaced with electrospray mass spectrometry. Changes in brain monoamines were measured by HPLC with electrochemical detection, and serum corticosterone and leptin were monitored using radioimmunoassay. Treatment with PFOS produced a dose-dependent accumulation of this chemical in various body tissues, including the brain. PFOS exposure decreased food intake and BW in a dose-dependent manner. Treatment with PFOS affected estrous cyclicity and increased serum corticosterone levels while decreasing serum leptin concentrations. PFOS treatment also increased norepinephrine concentrations in the paraventricular nucleus of the hypothalamus. These results indicate that exposure to PFOS can affect the neuroendocrine system in rats.