Identification of key taxa that favor intestinal colonization of Clostridium difficile in an adult Chinese population
Identification of key taxa that favor intestinal colonization of Clostridium difficile in an adult Chinese population
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DOI：
10.1016/j.micinf.2015.09.008
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发表时间：
2016-01-01
影响因子：
5.8
通讯作者：
Li, Lanjuan
中科院分区：
文献类型：
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作者：
Gu, Silan;Chen, Yunbo;Li, Lanjuan
粪便微生物移植为复发性艰难梭菌感染（CDI）提供了高治愈率。然而，与FMT相关的局限性导致需要确定用于选择性益生菌疗法的关键分类群，以预防、治疗和治愈人类CDI。在西方国家的成年患者和中国的婴儿人群中研究了CDI相关的肠道微生物群变化。然而，在中国还没有涉及成年患者的此类研究。因此，使用16S核糖体RNA V3区的高通量测序和实时定量聚合酶链反应，我们通过比较15名CDI成人患者和18名C.艰难梭菌阴性医院腹泻（CDN）和25名健康对照受试者。与健康对照组相比，CDI和CDN组的粪便细菌多样性减少，肠道微生物组成发生显著变化。与健康对照组相比，CDI患者中推定的产丁酸盐厌氧菌显著减少，而产内毒素的机会致病菌和产乳酸盐的厌氧菌类型显著增加。因此，有必要进一步筛选引起烟草病害的特异性微生物和对CDI的抗性。(C)2015巴斯德研究所。由Elsevier Masson SAS出版。All rights reserved.
Fecal microbial transplantation provides a high curative rate for recurrent Clostridium difficile infection (CDI). However, limitations associated with FMT drive the need to identify key taxa for selective probiotic therapy for prevention, treatment and cure of human CDI. CDI-associated changes in gut microbiota were investigated in adult patients in the Western countries and among infant population in China. However, there has been no such study involving adult patients in China. Therefore, using high throughput sequencing of the 16S ribosomal RNA V3 region and real-time quantitative polymerase chain reaction, we identified CDI-associated key taxa by comparing the fecal microbiota composition of 15 adult patients with CDI with those of 18 individuals with C. difficile-negative nosocomial diarrhea (CDN) and 25 healthy control subjects. Reduced fecal bacterial diversity and dramatic shifts of intestinal microbial composition in CDI and CDN groups were observed compared with healthy controls. Putative butyrate-producing anaerobic bacteria were significantly depleted whereas endotoxin-producing opportunistic pathogens and lactate-producing phylotypes increased dramatically in patients with CDI compared with healthy controls. Further screening of specific microbes causing diarrheal diseases and resistance against CDI is necessary. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.