Evaluation of 2 Ultraviolet-C Light Boxes for Decontamination of N95 Respirators.
Evaluation of 2 Ultraviolet-C Light Boxes for Decontamination of N95 Respirators.
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DOI：
10.20411/pai.v6i1.432
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发表时间：
2021
影响因子：
--
通讯作者：
Donskey CJ
中科院分区：
文献类型：
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作者：
Cadnum JL;Pearlmutter BS;Li DF;Jencson AL;Scott JG;Charnas IC;Donskey CJ
紫外线- c （UV-C）光装置可有效减少N95过滤式口罩上的污染。然而，关于UV-C设备是否符合美国食品和药物管理局（FDA）紧急使用授权（EUA）对呼吸器生物负荷减少的微生物要求的信息有限。我们测试了2个UV-C灯箱的能力，以达到单个用户重复使用EUA所需的3-log10微生物减少量。用噬菌体MS2和/或4株细菌接种整个3M 1860或Moldex 1513口罩于面罩的外部、内部和内部纤维上，用UV-C循环处理1或20分钟。比色指标用于评估紫外线- c穿透呼吸器的能力。对于1个UV-C盒，20分钟的处理达到了Moldex 1513口罩所需的生物负荷降低，但3M 1860口罩不能。对于第二个UV-C盒，1分钟的处理达到了Moldex 1513呼吸器所需的4种细菌菌株的生物负荷降低。比色指标表明，UV-C可以穿透Moldex 1513口罩的所有层，但3M 1860口罩却不能。我们的研究结果表明，UV-C盒技术可以达到FDA对单个用户EUA的生物负荷降低要求，但突出了不同类型呼吸器的不同功效的潜力。
Ultraviolet-C (UV-C) light devices are effective in reducing contamination on N95 filtering facepiece respirators. However, limited information is available on whether UV-C devices meet the Food and Drug Administration's (FDA) microbiological requirements for Emergency Use Authorization (EUA) for respirator bioburden reduction. We tested the ability of 2 UV-C light boxes to achieve the 3-log10 microorganism reductions required for EUA for reuse by single users. Whole 3M 1860 or Moldex 1513 respirators were inoculated on the exterior facepiece, interior facepiece, and internal fibers with bacteriophage MS2 and/or 4 strains of bacteria and treated with UV-C cycles of 1 or 20 minutes. Colorimetric indicators were used to assess penetration of UV-C through the respirators. For 1 UV-C box, a 20-minute treatment achieved the required bioburden reduction for Moldex 1513 but not 3M 1860 respirators. For the second UV-C box, a 1-minute treatment achieved the required bioburden reduction in 4 bacterial strains for the Moldex 1513 respirator. Colorimetric indicators demonstrated penetration of UV-C through all layers of the Moldex 1513 respirator but not the 3M 1860 respirator. Our findings demonstrate that UV-C box technologies can achieve bioburden reductions required by the FDA for EUA for single users but highlight the potential for variable efficacy for different types of respirators.