Engineering a Novel 3D Printed Vascularized Tissue Model for Investigating Breast Cancer Metastasis to Bone
Engineering a Novel 3D Printed Vascularized Tissue Model for Investigating Breast Cancer Metastasis to Bone
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DOI：
10.1002/adhm.201900924
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发表时间：
2019-12-17
影响因子：
10
通讯作者：
Zhang, Lijie Grace
中科院分区：
文献类型：
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作者：
Cui, Haitao;Esworthy, Timothy;Zhang, Lijie Grace
由于其器官特异性和病理生理的复杂性，癌症转移是癌症治疗的一个挑战。设计能够复制自然癌症扩散的3D体外模型可以显著提高对癌症生物学的理解，并有助于指导更有效的治疗方法的开发。为了更好地模拟自然癌症的行为，使用立体光刻印刷技术和优化的油墨创建了三种培养转移模型，以研究乳腺癌(BRCA)细胞对带血管的骨组织的侵袭。打印的系统允许研究转移的BRCA细胞的跨内皮迁移和集落形成行为。连续监测BRCA细胞发展的关键步骤，包括扩增、迁移和定植，并系统地研究癌细胞、血管细胞和骨细胞之间的相互作用。研究结果表明，由多个细胞和各种有利的墨水基质构建的3D打印组织为研究复杂血管微环境中这些细胞之间的相互作用提供了一个合适的模型。因此，3D打印的三元培养模型可能成为研究转移性乳腺癌骨进展的有价值的工具。
Cancer metastases are a challenge for cancer treatment due to their organ specificity and pathophysiological complexity. Engineering 3D in vitro models capable of replicating native cancer dissemination can significantly improve the understanding of cancer biology and can help to guide the development of more effective treatments. In order to better mimic the behavior of native cancer, a triculture metastatic model is created using a stereolithography printing technique with optimized inks for investigating the invasion of breast cancer (BrCa) cells into vascularized bone tissue. The printed system allows to study transendothelial migration and the colony-forming behavior of metastatic BrCa cells. The key steps of BrCa cell progression including expansion, migration, and colonization are continuously monitored and the interactions between cancer cells, vascular cells, and bone cells are systematically investigated. The study results demonstrate that the 3D printed tissue construct by incorporating multiple cells and various favorable ink matrices provides a suitable model to study the interaction between these cells in a complex vascular microenvironment. As such, the 3D printed tricultured model may serve as a valuable tool for studying metastatic breast cancer progression in bone.