Astragalus polysaccharides: An effective treatment for diabetes prevention in NOD mice
Astragalus polysaccharides: An effective treatment for diabetes prevention in NOD mice
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DOI：
10.1055/s-2008-1058081
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发表时间：
2008-08-01
影响因子：
1.8
通讯作者：
Yu, M. -H.
中科院分区：
文献类型：
--
作者：
Chen, W.;Li, Y. -M.;Yu, M. -H.
背景资料：1型糖尿病（Type 1 Diabetes Mellitus，DM）是一种与辅助性T淋巴细胞（Helper T lymphocyte，Th）Th 1、Th 2亚群及其细胞因子失衡有关的慢性自身免疫性疾病。黄芪多糖（Astragalus polysaccharides，APS）是从传统中药中提取的一种生物活性成分，具有增强免疫功能的作用。目的：比较黄芪多糖和生理盐水对非肥胖糖尿病（non-obese diabetes，NOD）小鼠的干预作用，探讨黄芪多糖对I型糖尿病的预防作用及对Th 1/Th 2细胞因子的影响。APS或NS以2.0g/kg/d的剂量给予4周龄小鼠，连续10周。40周时检测血糖、血清C肽（C-P）、GAD抗体，胰腺组织学检查，胰岛内淋巴细胞浸润及脾脏T淋巴细胞亚群，免疫组化染色观察胰腺病理变化。IL-1 β，IL-2，IL-6，IL-12，TNF-α，INF-γ，IL-4，IL-5，IL-10，TGF-β，Bcl-2，SOD，结果：APS组NOD小鼠1型糖尿病发病率较低，血清C-肽水平较低，胰岛组织学改变较好，脾脏和胰岛浸润T淋巴细胞D4+/CD 8+比值较低。RT-PCR分析显示，与NS组相比，APS给药NOD小鼠胰腺组织中IL-1 β、IL-2、IL-6、IL-12、TNF-α、INF-γ、Fas、iNOS的基因表达水平较低，而IL-4、IL-5、IL-10、TGF-β、Bcl-2、SOD的基因表达水平较高。这些结果表明黄芪多糖通过纠正Th 1/Th 2细胞因子之间的失衡来预防NOD小鼠的1型糖尿病。
Background: Type 1 diabetes mellitus (DM) is a chronic autoimmune disease that is related to the disequilibrium state of Th1 and Th2 subgroups of helper T lymphocyte (Th) and their cytokines. Astragalus polysaccharides (APS) are bioactive components extracted from one of the traditional Chinese herbs, used to enhance the function of human immune system.Objective: To investigate the effects of APS on preventing type I DM and Th1/Th2-subtype cytokines, we compared the results of administration of APS and normal saline (NS) on non-obese diabetic (NOD) mice.Subjects and Methods: APS or NS was administered to 4-week-old mice at a dose of 2.0g/kg per day for 10 weeks. At 40 weeks, blood glucose, serum C-peptide (C-P) and GAD antibody were measured; pancreas was examined histologically; the intra-islet lymphocyte infiltration and T lymphocyte subsets in the spleen were analysed; the gene expression of IL-1 beta, IL-2, IL-6, lL-12,TNF-alpha, INF-gamma, IL-4, IL-5, IL-10, TGF-beta, Bcl-2, SOD, Fas and iNOS were measured by RT-PCR.Results: The results showed that APS-administered NOD mice had a lower incidence rate of type 1 DM, lower serum C-P level, better histologic findings of pancreatic islets, and a lower D4+/CD8+ ratio of T lymphocytes from the spleen and the infiltrated islets. RT-PCR analysis showed gene expression levels are lower in IL-1 beta, IL-2, IL-6, IL-12, TNF-alpha, INF-gamma, Fas, iNOS, and higher in IL-4, IL-5, IL-10, TGF-beta, Bcl-2, SOD in the pancreatic tissue from APS-administered NOD mice as compared to the NS group.Conclusions: These results demonstrated the effects of Astragalus polysaccharides on the prevention of type 1 DM in NOD mice by correcting the imbalance between the Th1/Th2 cytokines.