Quantum-dot based nanoparticles for targeted silencing of HER2/neu gene via RNA interference
Quantum-dot based nanoparticles for targeted silencing of HER2/neu gene via RNA interference
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DOI：
10.1016/j.biomaterials.2006.11.018
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发表时间：
2007-03-01
期刊：
影响因子：
14
通讯作者：
Zhang, Yong
中科院分区：
文献类型：
--
作者：
Tan, Wee Beng;Jiang, Shan;Zhang, Yong
使用短干扰RNA（siRNA）的基因沉默正迅速成为在哺乳动物细胞中探测基因功能的有吸引力的方法。尽管使用各种方法在siRNA的递送中已经取得了一些成功，但是在没有合适的追踪剂的情况下，追踪它们的递送和监测它们的转染效率被证明是困难的。因此，挑战在于设计用于RNA干扰的有效且同时自跟踪的转染剂。本文合成了壳聚糖纳米粒（Nanoparticles，NPs）包裹量子点（Quantum Dots，QDs），并将其用于HER 2/neu siRNA的递送。使用这样的构建体，siRNA的递送和转染可以通过壳聚糖NP中荧光QD的存在来监测。HER 2 siRNA向HER 2过表达SKBR 3乳腺癌细胞的靶向递送被证明是特异性的，用靶向SKBR 3细胞上的HER 2受体的HER 2抗体标记的壳聚糖/QD NP表面。还使用荧光素酶和HER 2 ELISA测定确定了缀合的siRNA的基因沉默效应。这些自跟踪siRNA递送NP也将有助于监测未来的体内基因沉默研究。(c)2006爱思唯尔有限公司保留所有权利。
Gene silencing using short interfering RNA (siRNA) is fast becoming an attractive approach to probe gene function in mammalian cells. Although there have been some success in the delivery of siRNA using various methods, tracking their delivery and monitoring their transfection efficiency prove to be hard without a suitable tracking agent. Therefore, a challenge lies with the design of an efficient and at the same time, self-tracking, transfection agent for RNA interference. In this paper, chitosan nanoparticles (NPs) with encapsulated quantum dots (QDs) were synthesized and used to deliver HER2/neu siRNA. Using such a construct, the delivery and transfection of the siRNA can be monitored by the presence of fluorescent QDs in the chitosan NPs. Targeted delivery of HER2 siRNA to HER2-overexpressing SKBR3 breast cancer cells was shown to be specific with chitosan/QD NP surface labeled with HER2 antibody targeting the HER2 receptors on SKBR3 cells. Gene-silencing effects of the conjugated siRNA was also established using the luciferase and HER2 ELISA assays. These self-tracking siRNA delivery NPs will also aid in the monitoring of future gene silencing studies in vivo. (c) 2006 Elsevier Ltd. All rights reserved.