Degree of Biomimicry of Artificial Spider Silk Spinning Assessed by NMR Spectroscopy.
Degree of Biomimicry of Artificial Spider Silk Spinning Assessed by NMR Spectroscopy.
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DOI：
10.1002/anie.201706649
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发表时间：
2017-10
影响因子：
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通讯作者：
M. Otikovs;M. Andersson;Qiupin Jia;K. Nordling;Qing Meng;L. Andreas;G. Pintacuda;J. Johansson;A. Rising;K. Jaudzems
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文献类型：
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作者：
M. Otikovs;M. Andersson;Qiupin Jia;K. Nordling;Qing Meng;L. Andreas;G. Pintacuda;J. Johansson;A. Rising;K. Jaudzems
人造蜘蛛丝的仿生纺丝需要设计的迷你蛛丝体的末端结构域经历特定的结构变化，以与重复区域的β-Sheet转换相一致。在这里，我们结合溶液和固体核磁共振方法来探索NT2RepCT minispidroin中的结构域特异性结构变化，这使我们能够评估人造丝纺纱的仿生程度。此外，我们还证明了后自旋过程的结构效应是可以检验的。通过研究NT2RepCT纤维干燥的影响，我们观察到了可逆的β-α转化。我们认为，这一方法将有助于指导人造蜘蛛丝纤维的优化。
Biomimetic spinning of artificial spider silk requires that the terminal domains of designed minispidroins undergo specific structural changes in concert with the β-sheet conversion of the repetitive region. Herein, we combine solution and solid-state NMR methods to probe domain-specific structural changes in the NT2RepCT minispidroin, which allows us to assess the degree of biomimicry of artificial silk spinning. In addition, we show that the structural effects of post-spinning procedures can be examined. By studying the impact of NT2RepCT fiber drying, we observed a reversible beta-to-alpha conversion. We think that this approach will be useful for guiding the optimization of artificial spider silk fibers.