Advanced backcross QTL analysis for the identification of quantitative trait loci alleles from wild relatives of wheat (Triticum aestivum L.)
Advanced backcross QTL analysis for the identification of quantitative trait loci alleles from wild relatives of wheat (Triticum aestivum L.)
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DOI：
10.1007/s00122-002-1179-7
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发表时间：
2003-05-01
影响因子：
5.4
通讯作者：
Roeder, M. S.
中科院分区：
文献类型：
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作者：
Huang, X. Q.;Coester, H.;Roeder, M. S.
以德国冬小麦品种Prinz与CIMMYT人工合成小麦品系W-7984杂交的BC 2F 2群体为材料，利用先进的回交QTL（AB-QTL）分析方法，对产量及其构成因素进行了QTL定位。采用210个微卫星标记对72个预选的BC 2F 2植株进行基因分型，并从生长在德国4个地点的相应BC 2F 3家系收集5个农艺性状的表型数据。利用单标记回归和区间作图方法，共检测到40个来自W-7984的QTL，其中11个与产量相关，16个与产量构成因素相关，8个与抽穗期相关，5个与株高相关。对于其中的24个（60.0%），来自合成小麦W-7984的等位基因与对农艺性状的积极影响相关，尽管事实上合成小麦在农艺外观和性能方面总体较差。本研究表明，利用先进的回交QTL策略和分子育种技术，可以将小麦近缘野生种的有利QTL等位基因转移到优良小麦品种中，用于产量等数量性状的改良。据我们所知，本研究结果是小麦AB-QTL分析的首次报道。
Advanced backcross QTL (AB-QTL) analysis was used to identify quantitative trait loci (QTLs) for yield and yield components in a BC2F2 population derived from a cross between the German winter wheat variety 'Prinz' and the synthetic wheat line W-7984 developed by CIMMYT. Two hundred and ten microsatellite markers were employed to genotype 72 pre-selected BC2F2 plants and phenotypic data were collected for five agronomic traits from corresponding BC2F3 families that were grown at four locations in Germany. Using single-marker regression and interval mapping, a total of 40 putative QTLs derived from W-7984 were detected, of which 11 were for yield, 16 for yield components, eight for ear emergence time and five for plant height. For 24 (60.0%) of them, alleles from the synthetic wheat W-7984 were associated with a positive effect on agronomic traits, despite the fact that synthetic wheat was overall inferior with respect to agronomic appearance and performance. The present study indicated that favorable QTL alleles could be transferred from wild relatives of wheat into an elite wheat variety for improvement of quantitative trait loci like yield by the advanced backcross QTL strategy and molecular breeding. To our knowledge, the results presented here were the first report on AB-QTL analysis in wheat.