Rapid cancer diagnosis by highly fluorescent carbon nanodots-based imaging
Rapid cancer diagnosis by highly fluorescent carbon nanodots-based imaging
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DOI：
10.1007/s00216-018-1500-1
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发表时间：
2019-02-01
影响因子：
4.3
通讯作者：
Sang, Shengbo
中科院分区：
文献类型：
--
作者：
Duan, Qianqian;Che, Mingxuan;Sang, Shengbo
具有亮绿色荧光的碳点（Cdots）被应用于多种细胞系的快速选择性细胞成像。使用碳点作为显像剂实现了肝癌细胞 (HepG2) 和正常人肝细胞 (LO2) 的不同标记分布。对于HepG2细胞，碳点可以在3分钟内快速渗透细胞膜并扩散到细胞质和细胞核中，并在靶标中保留其位置24小时。然而，碳点仅在 LO2 细胞系的细胞质中表现出明亮的荧光。此外，碳点几乎无细胞毒性，并且在较宽的 pH 范围内表现出优异的光稳定性。因此，这些碳点在快速、发光和选择性生物成像应用方面具有巨大潜力，有望用作癌症诊断中的细胞核染色剂。
Carbon dots (Cdots) with bright green fluorescence were applied to the rapid and selective cell imaging for a variety of cell lines. Different labeling distributions of hepatoma cells (HepG2) and normal human liver cells (LO2) were achieved using Cdots as imaging agents. For HepG2 cells, the Cdots could rapidly permeate the cell membrane and diffuse into the cytoplasm and nucleus within 3min, and retained their location in the targets for 24h. However, the Cdots exhibited bright fluorescence only in the cytoplasm of LO2 cell lines. Moreover, the Cdots were almost non-cytotoxic and exhibited superior photostability over a wide range of pH. Therefore, these Cdots have great potential for rapid, luminous and selective bioimaging applications, and are expected to be used as a nucleus-staining agent in cancer diagnosis.