A review of the methods for human iPSC derivation.
A review of the methods for human iPSC derivation.
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DOI：
10.1007/978-1-62703-348-0_3
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Rao, Mahendra S
中科院分区：
文献类型：
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作者：
Malik, Nasir;Rao, Mahendra S
将体细胞重编程为诱导多能干细胞（iPSC）的能力提供了产生多能患者特异性细胞系的机会，这些细胞系可以帮助模拟人类疾病。这些iPSC细胞系也可能成为药物发现和细胞移植疗法开发的有力工具。存在许多用于产生iPSC系的方法，但最适合用于研究人类疾病和开发疗法的那些方法必须具有足够的效率，以从可能丰度有限的样品产生iPSC，能够从皮肤成纤维细胞和血液重新编程细胞，并且没有足迹。几种重编程技术符合这些标准，可以用于在具有基本科学和治疗目标的项目中获得iPSC。将这些重编程方法与信号传导途径的小分子调节剂组合可以导致甚至从最不稳定的患者来源的体细胞成功产生iPSC。
The ability to reprogram somatic cells to induced pluripotent stem cells (iPSCs) offers an opportunity to generate pluripotent patient-specific cell lines that can help model human diseases. These iPSC lines could also be powerful tools for drug discovery and the development of cellular transplantation therapies. Many methods exist for generating iPSC lines but those best suited for use in studying human diseases and developing therapies must be of adequate efficiency to produce iPSCs from samples that may be of limited abundance, capable of reprogramming cells from both skin fibroblasts and blood, and footprint-free. Several reprogramming techniques meet these criteria and can be utilized to derive iPSCs in projects with both basic scientific and therapeutic goals. Combining these reprogramming methods with small molecule modulators of signaling pathways can lead to successful generation of iPSCs from even the most recalcitrant patient-derived somatic cells.