Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
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DOI：
10.1111/j.1365-313x.2012.05049.x
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发表时间：
2012-10-01
期刊：
影响因子：
7.2
通讯作者：
Wenkel, Stephan
中科院分区：
文献类型：
--
作者：
Brandt, Ronny;Salla-Martret, Merce;Wenkel, Stephan
与动物的情况不同，植物体的最终形态受到环境的高度调节。在拟南芥的发育过程中，内在因子为基本的模式化过程提供了框架。III类同源域亮氨酸拉链（HD-ZIPIII）转录因子参与胚、芽和根的图案形成。在营养生长期间，HD-ZIPIII蛋白控制几个极性建立过程，例如在叶片和维管系统中。我们使用染色质免疫沉淀/DNA测序（ChIP-Seq）方法鉴定了HD-ZIPIII转录因子REVOLUTA（REV）的几个直接靶基因。该分析表明，REV作用于生长素生物合成的上游，并直接影响已被证明在避荫反应途径中起作用的几个II类HD-ZIP转录因子的表达。我们表明，以及参与基本图案，HD-ZIPIII转录因子在控制植物经历遮荫时诱导的伸长生长中起着关键作用。叶极性是由HD-ZIPIII和KANADI转录因子的相反作用建立的。最后，我们的研究表明，由HD-ZIPIII/KANADI转录因子组成的模块拮抗性地控制遮荫生长，并且这种拮抗作用表现在共享靶基因的相反调节中。
Unlike the situation in animals, the final morphology of the plant body is highly modulated by the environment. During Arabidopsis development, intrinsic factors provide the framework for basic patterning processes. CLASS III HOMEODOMAIN LEUCINE ZIPPER (HD-ZIPIII) transcription factors are involved in embryo, shoot and root patterning. During vegetative growth HD-ZIPIII proteins control several polarity set-up processes such as in leaves and the vascular system. We have identified several direct target genes of the HD-ZIPIII transcription factor REVOLUTA (REV) using a chromatin immunoprecipitation/DNA sequencing (ChIP-Seq) approach. This analysis revealed that REV acts upstream of auxin biosynthesis and affects directly the expression of several class II HD-ZIP transcription factors that have been shown to act in the shade-avoidance response pathway. We show that, as well as involvement in basic patterning, HD-ZIPIII transcription factors have a critical role in the control of the elongation growth that is induced when plants experience shade. Leaf polarity is established by the opposed actions of HD-ZIPIII and KANADI transcription factors. Finally, our study reveals that the module that consists of HD-ZIPIII/KANADI transcription factors controls shade growth antagonistically and that this antagonism is manifested in the opposed regulation of shared target genes.