Immune checkpoint expression in HNSCC patients before and after definitive chemoradiotherapy
Immune checkpoint expression in HNSCC patients before and after definitive chemoradiotherapy
复制标题
DOI：
10.1002/hed.26534
复制
发表时间：
2020-11-06
影响因子：
2.9
通讯作者：
Weissinger, Stephanie Ellen
中科院分区：
文献类型：
--
作者：
Doescher, Johannes;Minkenberg, Peter;Weissinger, Stephanie Ellen
背景原发性铂基放化疗（CRT）仍然是不可切除的头颈部鳞状细胞癌（HNSCC）的首选治疗方法。免疫检查点调节剂被用作姑息治疗，并与明确的CRT联合研究。然而，CRT对免疫系统的影响尚不清楚。方法对67例HNSCC患者（N = 134）在CRT前后进行配对组织活检。免疫组化法检测肿瘤细胞和免疫细胞PD-1、PD-L1、CD27的表达。结果无应答者免疫细胞PD-L1表达明显低于CRT前（P = 0.008）。CD27仅在免疫细胞上表达，而不在癌细胞上表达。CRT后cd27表达评分明显降低（P = 0.019）。结论常规CRT可改变肿瘤微环境中CD27的表达。这是由于表达缺失还是CD27+细胞的减少，必须在进一步的分析中进行评估。
Background Primary platinum-based chemoradiotherapy (CRT) remains the treatment of choice for nonresectable squamous cell carcinoma of the head and neck (HNSCC). Immune-checkpoint modulators are used as palliative therapy and studied in combination with definitive CRT. However, the immunological changes by CRT need yet to be understood.Methods A cohort consisting of 67 paired tissue biopsies (N = 134) of HNSCC patients before and after CRT was created. The expression of PD-1, PD-L1, and CD27 of tumor and immune cells by immunohistochemistry was evaluated.Results PD-L1 expression on immune cells of non-responders was significantly lower before CRT (P = .008). CD27 was expressed only on immune cells and not on cancer cells. A significant lower CD27-expression score was observed following CRT (P = .019).Conclusions Conventional CRT changes the expression of CD27 in the tumor microenvironment. Whether this is due to a loss of expression or a reduction of CD27+ cells must be evaluated in further analyses.