Specific receptor-mediated inhibition by synthetic atrial natriuretic factor of hormone-stimulated steroidogenesis in cultured bovine adrenal cells.
Specific receptor-mediated inhibition by synthetic atrial natriuretic factor of hormone-stimulated steroidogenesis in cultured bovine adrenal cells.
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合成心房钠尿因子对培养的牛肾上腺细胞中激素刺激的类固醇生成的特异性受体介导的抑制。
作者：
A. De Léan;K. Rácz;J. Gutkowska;T. T. Nguyen;M. Cantin;J. Genest
在原代培养的牛肾上腺细胞中研究了合成心钠素（ANF）对肾上腺类固醇生成的影响。ANF-（8-33）对血管紧张素II、ACTH、PGE 1和毛喉素刺激的肾小球内皮细胞分泌醛固酮产生40-70%的有效抑制，ED 50为120 pM。利钠因子对皮质醇产生的等效抑制作用也观察到在培养的神经纤维细胞。尼古丁刺激分泌的儿茶酚胺从髓细胞仅轻微抑制的因素在剂量高于10 nM。[125 I]碘-ANF-（8-33）与肾小球膜的结合显示出对特异性受体位点的100-150 pM的表观亲和力，并且不受血管紧张素II或ACTH的抑制。相反，利钠因子对血管紧张素II受体位点没有亲和力。结果表明，这种新因子的利钠利尿作用的一部分可能是由于通过一个不同的受体作用抑制肾上腺类固醇合成。
The effect of synthetic atrial natriuretic factor (ANF) on adrenal steroidogenesis has been studied in primary culture of bovine adrenal cells. ANF-(8-33) produced a potent 40-70% inhibition of angiotensin II-, ACTH-, PGE1-, and forskolin-stimulated secretion of aldosterone production from zona glomerulosa cells with an ED50 of 120 pM. An equipotent inhibitory effect of the natriuretic factor on cortisol production was also observed in cultured zona fasciculata cells. Nicotine-stimulated secretion of catecholamines from medullary cells was only slightly inhibited by the factor at doses above 10 nM. [125I]iodo-ANF-(8-33) binding to glomerulosa membranes displayed an apparent affinity of 100-150 pM for specific receptor sites and was not inhibited by angiotensin II or ACTH. Conversely, the natriuretic factor had no affinity for angiotensin II receptor sites. The results demonstrate that part of the natriuretic effect of this new factor might be due to inhibition of adrenal steroidogenesis by action through a distinct receptor.