Comparison of chromosomal DNA composing timeless in Drosophila melanogaster and D. virilis suggests a new conserved structure for the TIMELESS protein.
Comparison of chromosomal DNA composing timeless in Drosophila melanogaster and D. virilis suggests a new conserved structure for the TIMELESS protein.
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对果蝇和 D. virilis 中 TIMELESS 的染色体 DNA 组成的比较表明 TIMELESS 蛋白有一个新的保守结构。
DOI：
10.1093/nar/25.23.4710
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发表时间：
1997
影响因子：
14.9
通讯作者：
Young,MW
中科院分区：
文献类型：
--
作者：
Myers,MP;Rothenfluh,A;Chang,M;Young,MW
两种蛋白质TIM和PER通过物理相互作用来控制果蝇转录的昼夜节律。本研究通过分离和序列分析确定了D. virilis表达的TIM蛋白的结构。vtim和mtimgDNA的比较显示TIM蛋白高度保守。这与先前在这些物种中观察到的TIM伴侣蛋白PER的序列保守性差形成对比。vtimsequence的检查表明大多数TIM蛋白质的替代结构。在两个物种中，在先前描述的D.melanogastertimcDNA中形成内含子的序列似乎最常被翻译为产生较长的TIM蛋白。vTIM的N-末端序列和基因组DNA的序列分析从几个菌株ofD. melanogaster表明，只有一个两个可能的翻译起始位点发现intimmRNA是足以产生昼夜节律在D. melanogaster。TIM翻译可能受到多个AUG密码子的影响，这些密码子似乎在组成5′-未取代的timRNA前导序列中是保守的。
Two proteins, TIM and PER, physically interact to control circadian cycles oftimandpertranscription inDrosophila melanogaster. In the present study the structure of TIM protein expressed byD.viriliswas determined by isolation and sequence analysis of genomic DNA (gDNA) corresponding to theD.virilistimlocus (vtim). Comparison of vtimand mtimgDNA revealed high conservation of the TIM protein. This contrasts with poor sequence conservation previously observed for the TIM partner protein PER in these species. Inspection of the vtimsequence suggests an alternative structure for most TIM proteins. Sequences forming an intron in a previously characterizedD.melanogastertimcDNA appear to be most often translated to produce a longer TIM protein in both species. The N-terminal sequence of vTIM and sequence analysis of genomic DNA from several strains ofD.melanogastersuggest that only one of two possible translation initiation sites found intimmRNA is sufficient to generate circadian rhythms inD.melanogaster. TIM translation may be affected by multiple AUG codons that appear to have been conserved in sequences composing the 5′-untranslatedtimmRNA leader.