Large-scale temporal gene expression profiling during gonadal differentiation and early gametogenesis in rainbow trout
Large-scale temporal gene expression profiling during gonadal differentiation and early gametogenesis in rainbow trout
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DOI：
10.1095/biolreprod.105.041830
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发表时间：
2005-11-01
影响因子：
3.6
通讯作者：
Guiguen, Y
中科院分区：
文献类型：
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作者：
Baron, D;Houlgatte, R;Guiguen, Y
对脊椎动物性别分化级联的整体理解仍在缓慢增长，可能是因为使用的脊椎动物模型种类繁多，以及尚未发现的分子参与者的数量。在脊椎动物中发现保守的机制应该能更好地了解这一过程中涉及的关键因素。为此，我们利用实时逆转录聚合酶链反应，绘制了虹鳟鱼性别分化和配子发生早期102个基因mRNA表达波动的时序图。我们使用这102个时间基因表达模式作为分层聚类分析的基础，以找到共表达基因的特征聚类。对其中一些基因簇的分析表明，鱼类和哺乳动物性别分化级联的总体表达谱是保守的。例如，在这些保守的分子机制中，sox9、dmrt1、amh、nr5a1、nr0b1、igf1和igf1ra被认为是参与鳟鱼睾丸分化的早期表达基因，这是已知的或在哺乳动物中提出的。相反，foxl2、fst和lhr是在鳟鱼卵巢分化过程中早期表达的基因，在哺乳动物中也有发现。除了这种高度保守性之外，我们的分析还表明了一些潜在的新参与者，例如AM亚基基因，据我们所知，这是第一次在脊椎动物物种的雌性分化性腺中检测到的，它显示出与第一次卵母细胞减数分裂发生时间一致的特异性过表达，或者pax2基因，它显示出早期和睾丸特异性表达谱。
The overall understanding of the sex differentiation cascade in vertebrates is still growing slowly, probably because of the variety of vertebrate models used and the number of molecular players yet to be discovered. Finding conserved mechanisms among vertebrates should provide a better view of the key factors involved in this process. To this end, we used real-time reverse transcription-polymerase chain reaction to produce a temporal map of fluctuations in mRNA expression of 102 genes during sex differentiation and early gametogenesis in the rainbow trout (Oncorhynchus mykiss). We used these 102 temporal gene expression patterns as a basis for a hierarchical clustering analysis to find characteristic clusters of coexpressed genes. Analysis of some of these gene clusters suggested a conserved overall expression profile between the sex differentiation cascade in fish and mammals. Among these conserved molecular mechanisms, sox9, dmrt1, amh, nr5a1, nr0b1, igf1, and igf1ra are, for instance, characterized as early expressed genes involved in trout testicular differentiation as it is known or suggested in mammals. On the contrary, foxl2, fst, and lhr are characterized as early expressed genes during trout ovarian differentiation, as also found in mammals. Apart from this high conservation, our analysis suggests some potential new players, such as the AM subunit gene, which is detected here for the first time, to our knowledge, in the female differentiating gonad of a vertebrate species and displays a specific overexpression that coincides in timing with the occurrence of first oocyte meioses, or the pax2 gene, which displays an early and testis-specific expression profile.