Coexpression in yeast of Taxus cytochrome p450 reductase with cytochrorne P450 oxygenases involved in taxol biosynthesis
Coexpression in yeast of Taxus cytochrome p450 reductase with cytochrorne P450 oxygenases involved in taxol biosynthesis
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DOI：
10.1002/bit.20390
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发表时间：
2005-03-05
影响因子：
3.8
通讯作者：
Croteau, RB
中科院分区：
文献类型：
--
作者：
Jennewein, S;Park, H;Croteau, RB
为了最大限度地提高氧化还原偶联效率与重组细胞色素P450羟化酶从红豆杉（红豆杉）物种安装在酵母生产的抗癌药物紫杉醇，cDNA编码NADPH：细胞色素P450还原酶从T cuspidata分离。该单拷贝基因（2,154 bp，编码717个氨基酸的蛋白质）与裸子植物中的其他还原酶（相似性接近90%）比被子植物中的还原酶（
To maximize redox coupling efficiency with recombinant cytochrome P450 hydroxylases from yew (Taxus) species installed in yeast for the production of the anticancer drug Taxol, a cDNA encoding NADPH:cytochrome P450 reductase from T cuspidata was isolated. This single-copy gene (2,154 bp encoding a protein of 717 amino acids) resembles more closely other reductases from gymnosperms (similar to90% similarity) than those from angiosperms (