Molecular cloning and developmental expression of zinc finger transcription factor MTF-1 gene in zebrafish, Danio rerio
Molecular cloning and developmental expression of zinc finger transcription factor MTF-1 gene in zebrafish, Danio rerio
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DOI：
10.1006/bbrc.2002.6517
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发表时间：
2002-03-08
影响因子：
3.1
通讯作者：
Chang, CY
中科院分区：
文献类型：
--
作者：
Chen, WY;John, JAC;Chang, CY
金属响应转录因子MTF-1是一种锌指蛋白，对胚胎发育至关重要。MTF-1纯合子敲除小鼠胚胎在妊娠第14天死于子宫内，原因是肝脏腐烂。本研究报道了斑马鱼MTF-1基因的cDNA全序列，并分析了其与小鼠、人、鱼类和果蝇的氨基酸序列的相似性。斑马鱼MTF-1 cDNA的大小为3，379 bp，编码区（1，779 bp）编码593个氨基酸的多肽。还确定了斑马鱼MTF-1所包含的推定的锌指结构域和反式激活结构域。斑马鱼MTF-1与河豚、人、小鼠和果蝇的DNA结合锌指结构域的同源性分别为97%、93%、93%和67%，总体同源性分别为51%、44%、48%和20%。RTPCR结果显示了MTF-1转录本的母体表达。用地高辛标记的反义RNA探针，通过整体原位杂交技术研究了MTF-1基因在胚胎和早期幼虫发育过程中的表达模式。在胚胎阶段观察到更强和普遍的表达，而具体的表达，特别是在神经部分，观察到整个阶段研究孵化后。(C)2002 Elsevier Science（美国）。
Metal-responsive transcription factor, MTF-1 is a zinc finger protein, shown to be essential for embryonic development. Homozygous knockout mouse embryos for MTF-1 die in utero at day 14 of gestation, due to liver decay. In the present study, we report the complete nucleotide sequence of cDNA encoding zebrafish MTF-1 and the amino acid sequence similarity with that of mouse, human, fish and Drosophila. The size of the zebrafish MTF-1 cDNA is 3,379 bp and the coding region (1,779 bp) encodes a polypeptide of 593 amino acids. The putative zinc finger and transactivation domains comprised by zebrafish MTF-1 were also determined. The zebrafish MTF-1 shows high identity of 97, 93, 93 and 67% in the DNA binding zinc finger domain and 51, 44, 48 and 20% overall identity with fugu, human, mouse and Drosophila, respectively. RTPCR results show the maternal expression of MTF-1 transcripts. The pattern of MTF-1 gene expression during embryonic and early larval development was studied by whole-mount in situ hybridization using DIG-labeled anti-sense RNA probe. Stronger and ubiquitous expression was observed during the embryonic stages whereas, specific expression, especially in the neural parts, was observed throughout the stages studied after hatching. (C) 2002 Elsevier Science (USA).