Ultrasensitive specific stimulant assay based on molecularly imprinted photonic hydrogels
Ultrasensitive specific stimulant assay based on molecularly imprinted photonic hydrogels
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基于分子印迹光子水凝胶的超灵敏特异性兴奋剂测定
DOI：
10.1002/adfm.200700527
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发表时间：
2008-02-22
影响因子：
19
通讯作者：
Zhang, Yihe
中科院分区：
文献类型：
--
作者：
Hu, Xiaobin;Li, Guangtao;Zhang, Yihe
以茶碱和(1R,2S)-(-)-麻黄碱为模板分子，采用胶体晶体和分子印迹相结合的方法制备了两种印迹光子水凝胶膜。本文提出了一种快速简便、高灵敏度、高特异度的兴奋剂检测新方法。一种膜用于类似分子分析，另一种膜用于手性识别。该方法的关键在于，印迹光子聚合物（IPP）由一个三维（313）、高度有序和相互连接的大孔阵列和一个薄的水凝胶壁组成，其中纳米空腔在形状和结合位点上与被分析物互补。这种特殊的，双连续的，分层结构使这种聚合物能够快速，轻松，敏感和直接地报告分子识别事件，而无需任何传感器和分析物处理（无标签）。来自分子印迹的纳米空腔的固有亲和力使这些传感器即使在竞争环境中也能对分析物具有高度特异性。它们对缓冲介质中刺激物的敏感性和特异性反应是由Bragg衍射位移决定的，这是由于它们的三维有序大孔阵列的晶格变化导致它们优先与目标分子重新结合。测量结果表明，制备的水凝胶膜在0.1的温度下具有很高的灵敏度。分析物的fM浓度和特异性，即使在竞争性尿液缓冲液中。本方法为体育运动中的兴奋剂测定和药物分析提供了一种快速简便的方法。
Taking theophylline and (1R,2S)-(-)-ephedrine as template molecules, two imprinted photonic-hydrogel films are prepared by a combination of colloidal-crystal and molecular-imprinting techniques. This paper shows a new approach for rapid and handy stimulant detection with high sensitivity and specificity. One film is proposed for analogous molecule assay, another one for chiral recognition. The key point of this approach is that the imprinted photonic polymer (IPP) consists of a three-dimensional (313), highly-ordered and interconnected macroporous array with a thin hydrogel wall, where nanocavities complementary to analytes in shape and binding sites are distributed. This special, bicontinuous, hierarchical structure enables this polymer to report quickly, easily, sensitively and directly a molecular recognition event without any transducers and treatments for analytes (label-free). The I inherent affinity of the nanocavities, deriving from molecular imprinting, makes these sensors highly specific to analytes, even if in a competitive environment. Their sensitive and specific responses to stimulants in buffer are determined by Bragg diffractive shifts due to the lattice change of their 3D ordered macroporous arrays resulting from their preferential rebinding to the target molecules. The measurements show that the prepared hydrogel films exhibit high sensitivity in such a 0.1. fM concentration of analytes and specificity even in a competitive urinous buffer. The reported method provides a rapid and handy approach for stimulant assay and drug analysis in athletic sports.