Development of a Human Photoacoustic Imaging Reporter Gene Using the Clinical Dye Indocyanine Green
Development of a Human Photoacoustic Imaging Reporter Gene Using the Clinical Dye Indocyanine Green
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DOI：
10.1148/rycan.2019190035
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发表时间：
2019-11-01
期刊：
影响因子：
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通讯作者：
Ronald, John A.
中科院分区：
文献类型：
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作者：
Nystrom, Nivin N.;Yip, Lawrence C. M.;Ronald, John A.
目的：开发具有高翻译潜能的光声成像(PAI)报告基因。先前的研究表明，人有机阴离子转运多肽1b3(OATP1B3)能促进近红外荧光染料吲哚青绿(ICG)的摄取。在本研究中，作者建立了OATP1B3和ICG作为体内PAI的报告基因-探针对。材料和方法：利用基因工程技术在人乳腺癌细胞中表达OATP1B3。对照细胞(不表达OATP1B3)或表达OATP1B3的细胞与ICG孵育或不与ICG孵育，放置在模拟乳房的体模中，并用PAI成像。然后将对照组(n=6)或表达OATP1B3的细胞(n=5)原位移植到雌性小鼠体内。分别于ICG给药前和给药后24小时行全频谱PAI检查。结果：体外培养的OATP1B3细胞与ICG孵育后，OATP1B3表达细胞的对比度-噪声比比其他所有对照细胞高2.7倍(P
Purpose: To develop a photoacoustic imaging (PAI) reporter gene that has high translational potential. Previous research has shown that human organic anion-transporting polypeptide 1b3 (OATP1B3) promotes the uptake of the near-infrared fluorescent dye indocyanine green (ICG). In this study, the authors have established OATP1B3 and ICG as a reporter gene-probe pair for in vivo PAI.Materials and Methods: Human breast cancer cells were engineered to express OATP1B3. Control cells (not expressing OATP1B3) or OATP1B3-expressing cells were incubated with or without ICG, placed in a breast-mimicking phantom, and imaged with PAI. Control (n = 6) or OATP1B3-expressing (n = 5) cells were then implanted orthotopically into female mice. Full-spectrum PAI was performed before and 24 hours after ICG administration. One-way analysis of variance was performed, followed by Tukey posthoc multiple comparisons, to assess statistical significance.Results: OATP1B3-expressing cells incubated with ICG exhibited a 2.7-fold increase in contrast-to-noise ratio relative to all other controls in vitro (P