Heparin cross-linked collagen sponge scaffolds improve functional regeneration of rat tracheal epithelium.
Heparin cross-linked collagen sponge scaffolds improve functional regeneration of rat tracheal epithelium.
复制标题
肝素交联胶原海绵支架改善大鼠气管上皮的功能再生。
DOI：
10.1002/term.2204
复制
发表时间：
2017
影响因子：
3.3
通讯作者：
Omori K
中科院分区：
文献类型：
--
作者：
Nakamura R;Tani A;Yoshie S;Ikeda M;Wada I;Hazama A;Tada Y;Nomoto Y;Nakamura T;Omori K
气管上皮细胞通过介导粘膜纤毛清除维持气道内环境稳定。气管重建后，需要及时的上皮再生，以防止呼吸系统损害和感染性疾病。为了实现快速气管上皮再生，制备了含有成纤维细胞生长因子-2（FGF-2）的肝素交联胶原海绵作为气管重建的移植物。肝素交联海绵显示出高FGF-2保留能力，在含有FGF-2的海绵中培养的气管上皮细胞和间充质细胞显示出高增殖能力。随后，无肝素胶原海绵支架（C/F支架）和与10 μg/ml肝素交联的胶原海绵支架保留FGF-2（C/H10/F支架），并移植到气管缺损的大鼠中。在移植后1周，用C/H10/F支架处理的大鼠中上皮细胞和非上皮细胞的侵袭均大于用C/F支架处理的大鼠。此外，在移植后2周，在C/H10/F支架组中观察到改善的纤毛形成，具有比C/F支架组更高的运动性和更有效的后-前流动产生。这些结果表明，肝素改善气管上皮的功能性再生。版权所有© 2017约翰威利父子有限公司
Tracheal epithelial cells maintain airway homeostasis by mediating mucociliary clearance. Following tracheal reconstruction, timely epithelial regeneration is required to prevent respiratory compromise and infectious diseases. To achieve rapid tracheal epithelial regeneration, a heparin cross‐linked collagen sponge containing fibroblast growth factor‐2 (FGF‐2) was prepared as a graft for tracheal reconstruction. The heparin cross‐linked sponge exhibited a high FGF‐2 retaining capacity, and tracheal epithelial and mesenchymal cells cultured in this sponge containing FGF‐2 showed high proliferative capacities. Subsequently, heparin‐free collagen sponge scaffolds (C/F scaffold) and collagen sponge scaffolds cross‐linked with 10 μg/ml heparin retained FGF‐2 (C/H10/F scaffold), and were transplanted into rats with tracheal defects. Invasion of both epithelial and non‐epithelial cells was greater in rats treated with the C/H10/F scaffold at 1 week post‐transplantation than in rats treated with the C/F scaffold. Moreover, at 2 weeks after transplantation, improved cilia formation was observed in the C/H10/F scaffold group, with higher motility and more potent posterior–anterior flow generation than in the C/F scaffold group. These results suggest that heparin improves functional regeneration of tracheal epithelium. Copyright © 2017 John Wiley & Sons, Ltd.