Escherichia coli F-18 and E-coli K-12 eda mutants do not colonize the streptomycin-treated mouse large intestine
Escherichia coli F-18 and E-coli K-12 eda mutants do not colonize the streptomycin-treated mouse large intestine
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DOI：
10.1128/iai.64.9.3504-3511.1996
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发表时间：
1996-09-01
影响因子：
3.1
通讯作者：
Cohen, PS
中科院分区：
文献类型：
--
作者：
Sweeney, NJ;Laux, DC;Cohen, PS
大肠埃希菌人粪分离株F-18和K-12是链霉素处理的小鼠肠道的优良定植菌株，通过插入诱变获得了不能利用葡萄糖醛酸、半乳糖醛酸和葡萄糖酸的突变株F-18和K-12。E.coliF-18eda和E.coliK-12eda突变体都不能在链霉素处理的小鼠肠道内定植，无论是与它们各自的亲本菌株一起喂养还是单独喂给小鼠，与pTC190(含有功能性E.ColiK-12 eda基因)互补后，这两个eda突变体的定植能力都完全恢复了这两个eda突变体的定植能力。相对于它们的亲本菌株，E.coliF-18eda突变体和E.coliK-12eda突变体在饲喂正常小鼠饲料或以蔗糖为唯一碳源的合成饲料的小鼠盲肠粘液中生长不良。而突变株和亲本菌株在以葡萄糖为碳源的微量培养上的生长速度相同，大肠杆菌F-18EDD EDA和E.ColiK-12EDD EDA双突变株在链霉素处理的小鼠肠道中单独定植；然而，当它们与各自的亲本菌株同时饲养时，它们的定殖率很低，因为EDD基因只通过Entner-Doudoroff途径参与葡萄糖酸的代谢，这些结果表明葡萄糖酸和Entner-Doudoroff途径是链霉素处理的小鼠大肠在大肠杆菌定植中的重要元件。
The Escherichia coli human fecal isolates F-18 and K-12 are excellent colonizers of the streptomycin-treated mouse intestine, E. coli F-18 and E. coli K-12 eda mutants (unable to utilize glucuronate, galacturonate, and gluconate) were constructed by insertional mutagenesis. Neither the E. coli F-18 eda nor the E. coli K-12 eda mutant was able to colonize the streptomycin-treated mouse intestine, whether they were fed to mice together with their respective parental strains or alone, Complementation of the eda mutants with pTC190 (containing a functional E. coli K-12 eda gene) completely restored the colonization ability of both eda mutants, Relative to their parental strains, the E. coli F-18 eda mutant and the E. coli K-12 eda mutant grew poorly in cecal mucus isolated from mice fed either normal mouse chow or a synthetic diet containing sucrose as the sole carbon source, yet the mutants and parental strains demonstrated identical growth rates in minimal medium with glucose as the carbon source, E. coli F-18 edd eda and E. coli K-12 edd eda double mutants colonized the streptomycin-treated intestine when fed to mice alone; however, when fed simultaneously with their respective parental strains, they were poor colonizers, Since the edd gene is involved only in gluconate metabolism via the Entner-Doudoroff pathway, these results implicate the utilization of gluconate and the Entner-Doudoroff pathway as important elements in E. coli colonization of the streptomycin-treated mouse large intestine.