Germ cell-like telomeric length homeostasis in nonseminomatous testicular germ cell tumors
Germ cell-like telomeric length homeostasis in nonseminomatous testicular germ cell tumors
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DOI：
10.1038/sj.onc.1203746
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发表时间：
2000-08-17
期刊：
影响因子：
8
通讯作者：
Chrapusta, SJ
中科院分区：
文献类型：
--
作者：
Nowak, R;Sikora, K;Chrapusta, SJ
端粒维持在细胞增殖和肿瘤存活中起着重要作用。人类男性生殖细胞携带长端粒并表达端粒酶，产生一组高度异质性的恶性肿瘤。我们比较了原发性睾丸生殖细胞肿瘤的两种主要组织学类型之间的端粒长度和端粒酶活性。 16 个精原细胞瘤样本中有 15 个显示端粒限制性片段 (TRF) 长度低于 13 kb；其余的精原细胞瘤显示出 18 kb 的主要 TRF 部分和长度超过 23 kb 的明显次要部分。相比之下，来自非精原细胞瘤的所有 13 个样本均显示 TRF 长度大于或等于 23 kb，这与人类精子中报道的相似。研究的 11 个精原细胞瘤标本中的 9 个和 7 个非精原细胞瘤标本中的 6 个显示出中度至高端粒酶活性，唯一的端粒酶阴性非精原细胞瘤是纯成熟畸胎瘤。这些结果表明精原细胞瘤和非精原细胞瘤之间端粒长度的主要差异，这显然与端粒酶活性的存在无关，并表明人类非精原细胞瘤中保留了端粒长度的种系样稳态。
Telomere maintenance plays an important role in cell proliferation and tumor survival. Human male germ cells, which carry long telomeres and express telomerase, give rise to a highly heterogeneous group of malignant tumors. We compared telomeric length and telomerase activity between two major histological types of primary testicular germ cell tumors. Fifteen out of 16 seminoma samples revealed telomeric restriction fragment (TRF) length below 13 kb; the remaining seminoma showed a major TRF fraction of 18 kb and a distinct minor fraction of above 23 kb length. In contrast, all 13 samples from nonseminomas showed TRF length greater than or equal to 23 kb, which is similar to that reported in human sperm. Nine out of 11 seminoma specimens and six out of seven nonseminomas studied showed moderate to high telomerase activity, the only telomerase-negative non-seminoma being pure mature teratoma. These results indicate to a major difference in telomeric length between seminomas and nonseminomas, which is apparently unrelated to the presence of telomerase activity, and suggest a germline-like homeostasis of telomeric length is preserved in human nonseminomas.