Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies.
Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies.
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DOI：
10.3390/v10080436
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发表时间：
2018-08-17
期刊：
影响因子：
--
通讯作者：
Olmos A
中科院分区：
文献类型：
--
作者：
Maliogka VI;Minafra A;Saldarelli P;Ruiz-García AB;Glasa M;Katis N;Olmos A
多年生作物，如果树，被许多病毒感染，这些病毒通过受感染植物材料的营养繁殖和嫁接传播。这些病原体中的一些导致严重的作物损失，并经常减少果园的生产寿命。在果树中检测和表征这些试剂是具有挑战性的，然而，在过去的几年中，高通量测序（HTS）技术的广泛应用显着促进了这项任务。在这篇综述中，我们提出了最新的进展，发现，检测和表征果树病毒和病毒样剂完成HTS方法。在过去的5年中，已经描述了大量的新病毒，其中一些病毒表现出新的基因组特征，这导致了创建新属的建议，以及对当前病毒分类状态的修订。有趣的是，几种新鉴定的病毒属于以前未知的感染果树物种的病毒属（例如，Fabavirus，Luteovirus），这一事实挑战了我们对植物病毒的普遍看法。最后，应用方法，包括使用不同的分子作为模板，以及在果树病毒学HTS的优点和缺点和未来的发展方向进行了讨论。
Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted through vegetative propagation and grafting of infected plant material. Some of these pathogens cause severe crop losses and often reduce the productive life of the orchards. Detection and characterization of these agents in fruit trees is challenging, however, during the last years, the wide application of high-throughput sequencing (HTS) technologies has significantly facilitated this task. In this review, we present recent advances in the discovery, detection, and characterization of fruit tree viruses and virus-like agents accomplished by HTS approaches. A high number of new viruses have been described in the last 5 years, some of them exhibiting novel genomic features that have led to the proposal of the creation of new genera, and the revision of the current virus taxonomy status. Interestingly, several of the newly identified viruses belong to virus genera previously unknown to infect fruit tree species (e.g., Fabavirus, Luteovirus) a fact that challenges our perspective of plant viruses in general. Finally, applied methodologies, including the use of different molecules as templates, as well as advantages and disadvantages and future directions of HTS in fruit tree virology are discussed.
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影响因子：
3.2
作者：
Candresse, Thierry;Marais, Armelle;Gentit, Pascal
通讯作者：
Gentit, Pascal
影响因子：
3.2
作者：
Bag, Sudeep;Al Rwahnih, Maher;Sudarshana, Mysore R.
通讯作者：
Sudarshana, Mysore R.
影响因子：
2.7
作者：
Igori, Davaajargal;Lim, Seungmo;Moon, Jae Sun
通讯作者：
Moon, Jae Sun
影响因子：
3
作者：
Barrero RA;Napier KR;Cunnington J;Liefting L;Keenan S;Frampton RA;Szabo T;Bulman S;Hunter A;Ward L;Whattam M;Bellgard MI
通讯作者：
Bellgard MI
DOI：
10.5423/ppj.oa.04.2016.0104
发表时间：
2016-10
期刊：
The plant pathology journal
影响因子：
--
作者：
Cho IS;Igori D;Lim S;Choi GS;Hammond J;Lim HS;Moon JS
通讯作者：
Moon JS